tetano
Editor, Senior Moderator
Adv Healthc Mater. 2019 Aug 6:e1900575. doi: 10.1002/adhm.201900575. [Epub ahead of print]
[h=1]Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype.[/h] Tsang MK[SUP]1,[/SUP][SUP]2[/SUP], Wong YT[SUP]1[/SUP], Tsoi TH[SUP]2[/SUP], Wong WT[SUP]2[/SUP], Hao J[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, P. R. China. 2 Department of Applied Chemistry and Biology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, P. R. China.
[h=3]Abstract[/h] With large anti-Stokes shifts and background-free signals, upconversion luminescent (UCL) screening assays have been a promising method to reduce the transmission of influenza epidemic, which can critically alleviate the disease burden and extra annual deaths. In this work, a luminescent resonance energy transfer sandwich assay is developed, which utilizes core-shell upconversion nanoparticles and gold nanoparticles as the donor and acceptor, respectively. The influenza H7 gene of H7N9 virus is used as the target for optimization of the assay. Importantly, the hybridization time of the assay is ≈40 min and the specificity test indicates the probes are specific toward the H7 target. The limit of detection of the system is ≈134 ? 10[SUP]-12[/SUP] m (≈3.22 ? 10[SUP]10[/SUP] molecules). Moreover, the assay is tested with the use of polymerase chain reaction validated samples from human isolates. The results are promising for implanting future on-site rapid influenza screening application.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] DNA oligonucleotides; biodetections; bionanotechnologies; gold nanoparticles; upconversion nanoparticles
PMID: 31386308 DOI: 10.1002/adhm.201900575
[h=1]Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype.[/h] Tsang MK[SUP]1,[/SUP][SUP]2[/SUP], Wong YT[SUP]1[/SUP], Tsoi TH[SUP]2[/SUP], Wong WT[SUP]2[/SUP], Hao J[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, P. R. China. 2 Department of Applied Chemistry and Biology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, P. R. China.
[h=3]Abstract[/h] With large anti-Stokes shifts and background-free signals, upconversion luminescent (UCL) screening assays have been a promising method to reduce the transmission of influenza epidemic, which can critically alleviate the disease burden and extra annual deaths. In this work, a luminescent resonance energy transfer sandwich assay is developed, which utilizes core-shell upconversion nanoparticles and gold nanoparticles as the donor and acceptor, respectively. The influenza H7 gene of H7N9 virus is used as the target for optimization of the assay. Importantly, the hybridization time of the assay is ≈40 min and the specificity test indicates the probes are specific toward the H7 target. The limit of detection of the system is ≈134 ? 10[SUP]-12[/SUP] m (≈3.22 ? 10[SUP]10[/SUP] molecules). Moreover, the assay is tested with the use of polymerase chain reaction validated samples from human isolates. The results are promising for implanting future on-site rapid influenza screening application.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] DNA oligonucleotides; biodetections; bionanotechnologies; gold nanoparticles; upconversion nanoparticles
PMID: 31386308 DOI: 10.1002/adhm.201900575