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Vaccine . Immunogenicity and protective effects of recombinant bivalent COVID-19 vaccine in mice and rhesus macaques

tetano

Editor, Senior Moderator
Vaccine


. 2023 Jun 30;S0264-410X(23)00789-2.
doi: 10.1016/j.vaccine.2023.06.086. Online ahead of print. Immunogenicity and protective effects of recombinant bivalent COVID-19 vaccine in mice and rhesus macaques

Yuying Liu[SUP] 1 [/SUP], Aijing Zhang[SUP] 2 [/SUP], Yan Wang[SUP] 2 [/SUP], Jianguo Yang[SUP] 2 [/SUP], Fei Yin[SUP] 2 [/SUP], Shuming Wu[SUP] 2 [/SUP], Yao Zhang[SUP] 2 [/SUP], Xulin Jiang[SUP] 2 [/SUP], Jiaton Zhu[SUP] 2 [/SUP], Wenshuang Gao[SUP] 2 [/SUP], Xiufen Yang[SUP] 2 [/SUP], Hongyan Wen[SUP] 2 [/SUP], Qian Guo[SUP] 2 [/SUP], Xiao Chen[SUP] 2 [/SUP], Hongcai Zhang[SUP] 2 [/SUP], Ercui Shen[SUP] 2 [/SUP], Zengmin Yang[SUP] 2 [/SUP], Yakun Li[SUP] 2 [/SUP], Dan Chen[SUP] 2 [/SUP], Ling Li[SUP] 2 [/SUP], Jiaping Guo[SUP] 2 [/SUP], Xiaoli Du[SUP] 2 [/SUP], Yazheng Shi[SUP] 2 [/SUP], Sijia Fu[SUP] 2 [/SUP], Haijiang Zhang[SUP] 3 [/SUP], Yongjiang Liu[SUP] 4 [/SUP]



Affiliations
Abstract

Coronavirus disease (COVID-19) is still spreading rapidly worldwide, and a safe, effective, and cheap vaccine is still required to combat the COVID-19 pandemic. Here, we report a recombinant bivalent COVID-19 vaccine containing the RBD proteins of the prototype strain and beta variant. Immunization studies in mice demonstrated that this bivalent vaccine had far greater immunogenicity than the ZF2001, a marketed monovalent recombinant protein COVID-19 vaccine, and exhibited good immunization effects against the original COVID-19 strain and various variants. Rhesus macaque challenge experiments showed that this bivalent vaccine drastically decreased the lung viral load and reduced lung lesions in SARS-CoV-2 (the causative virus of COVID-19)-infected rhesus macaques. In summary, this bivalent vaccine showed immunogenicity and protective efficacy that was far superior to the monovalent recombinant protein vaccine against the prototype strain and provided an important basis for developing broad-spectrum COVID-19 vaccines.


 
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