tetano
Editor, Senior Moderator
J Med Virol. 2018 Dec 20. doi: 10.1002/jmv.25379. [Epub ahead of print]
[h=1]Viral Respiratory Infections Diagnosed by Multiplex PCR in Pediatric Patients.[/h] Appak ?[SUP]1[/SUP], Duman M[SUP]2[/SUP], Belet N[SUP]3[/SUP], Sayiner AA[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Syndromic diagnosis by multiplex nucleic acid amplification tests is the most practical approach to respiratory tract infections since the symptoms are rarely agent specific. The aim of this study was to investigate the respiratory viruses in children admitted to a university hospital with ARTI during the last 8 years by a multiplex PCR assay. A total of 3162 respiratory samples collected from children between April 2011 and April 2018 tested by a multiplex real-time PCR assay. Two different commercial assays were used during the study period, "AusDiagnostics/ Respiratory Pathogens 12 (Australia)" used between April 2011 and December 2015, which changed to "Fast Track Diagnostics/Respiratory Pathogens 21 (Luxemburg)" after January 2016 in order to cover more viruses. Nucleic acid extraction was done by EZ1 Advanced XL platform (Qiagen). Respiratory pathogens detected in 1857 of the 3162 (58.7%) samples. The most prevalent viruses during the 8-year period were rhino/enterovirus (RV/EV) (36.2%), respiratory syncytial virus (RSV) (19%), and influenza virus A/B (14.7%). Rhinovirus was the main contributor of the RV/EV group as shown by the assay used during 2016-2018 period. RV/EV and adenoviruses detected throughout the year. Influenza virus was most frequently detected during January - March when both RSV and metapneumovirus were also in circulation. The coinfection percentage was 10.2%. Rhinovirus was the most common virus in coinfections while RSV plus rhino/enterovirus were the most frequent combination. RSV and metapneumovirus showed a similar seasonal distribution to influenza virus, which made it necessary to use a virological diagnostic assay during influenza season. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Adult; Child; Multiplex Polymerase Chain Reaction; Respiratory Tract Infections; Viruses
PMID: 30570759 DOI: 10.1002/jmv.25379
[h=1]Viral Respiratory Infections Diagnosed by Multiplex PCR in Pediatric Patients.[/h] Appak ?[SUP]1[/SUP], Duman M[SUP]2[/SUP], Belet N[SUP]3[/SUP], Sayiner AA[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Syndromic diagnosis by multiplex nucleic acid amplification tests is the most practical approach to respiratory tract infections since the symptoms are rarely agent specific. The aim of this study was to investigate the respiratory viruses in children admitted to a university hospital with ARTI during the last 8 years by a multiplex PCR assay. A total of 3162 respiratory samples collected from children between April 2011 and April 2018 tested by a multiplex real-time PCR assay. Two different commercial assays were used during the study period, "AusDiagnostics/ Respiratory Pathogens 12 (Australia)" used between April 2011 and December 2015, which changed to "Fast Track Diagnostics/Respiratory Pathogens 21 (Luxemburg)" after January 2016 in order to cover more viruses. Nucleic acid extraction was done by EZ1 Advanced XL platform (Qiagen). Respiratory pathogens detected in 1857 of the 3162 (58.7%) samples. The most prevalent viruses during the 8-year period were rhino/enterovirus (RV/EV) (36.2%), respiratory syncytial virus (RSV) (19%), and influenza virus A/B (14.7%). Rhinovirus was the main contributor of the RV/EV group as shown by the assay used during 2016-2018 period. RV/EV and adenoviruses detected throughout the year. Influenza virus was most frequently detected during January - March when both RSV and metapneumovirus were also in circulation. The coinfection percentage was 10.2%. Rhinovirus was the most common virus in coinfections while RSV plus rhino/enterovirus were the most frequent combination. RSV and metapneumovirus showed a similar seasonal distribution to influenza virus, which made it necessary to use a virological diagnostic assay during influenza season. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Adult; Child; Multiplex Polymerase Chain Reaction; Respiratory Tract Infections; Viruses
PMID: 30570759 DOI: 10.1002/jmv.25379