tetano
Editor, Senior Moderator
Virus Evol
. 2020 Dec 30;7(1):veaa098.
doi: 10.1093/ve/veaa098. eCollection 2021 Jan.
Synonymous mutations and the molecular evolution of SARS-CoV-2 origins
Hongru Wang[SUP] 1 [/SUP], Lenore Pipes[SUP] 1 [/SUP], Rasmus Nielsen[SUP] 1 2 3 [/SUP]
Affiliations
Abstract
Human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is most closely related, by average genetic distance, to two coronaviruses isolated from bats, RaTG13 and RmYN02. However, there is a segment of high amino acid similarity between human SARS-CoV-2 and a pangolin-isolated strain, GD410721, in the receptor-binding domain (RBD) of the spike protein, a pattern that can be caused by either recombination or by convergent amino acid evolution driven by natural selection. We perform a detailed analysis of the synonymous divergence, which is less likely to be affected by selection than amino acid divergence, between human SARS-CoV-2 and related strains. We show that the synonymous divergence between the bat-derived viruses and SARS-CoV-2 is larger than between GD410721 and SARS-CoV-2 in the RBD, providing strong additional support for the recombination hypothesis. However, the synonymous divergence between pangolin strain and SARS-CoV-2 is also relatively high, which is not consistent with a recent recombination between them, instead, it suggests a recombination into RaTG13. We also find a 14-fold increase in the d[SUB]N[/SUB] /d[SUB]S[/SUB] ratio from the lineage leading to SARS-CoV-2 to the strains of the current pandemic, suggesting that the vast majority of nonsynonymous mutations currently segregating within the human strains have a negative impact on viral fitness. Finally, we estimate that the time to the most recent common ancestor of SARS-CoV-2 and RaTG13 or RmYN02 based on synonymous divergence is 51.71 years (95% CI, 28.11-75.31) and 37.02 years (95% CI, 18.19-55.85), respectively.
Keywords: SARS-CoV-2; molecular evolution; synonymous mutations.
. 2020 Dec 30;7(1):veaa098.
doi: 10.1093/ve/veaa098. eCollection 2021 Jan.
Synonymous mutations and the molecular evolution of SARS-CoV-2 origins
Hongru Wang[SUP] 1 [/SUP], Lenore Pipes[SUP] 1 [/SUP], Rasmus Nielsen[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 33500788
- PMCID: PMC7798566
- DOI: 10.1093/ve/veaa098
Abstract
Human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is most closely related, by average genetic distance, to two coronaviruses isolated from bats, RaTG13 and RmYN02. However, there is a segment of high amino acid similarity between human SARS-CoV-2 and a pangolin-isolated strain, GD410721, in the receptor-binding domain (RBD) of the spike protein, a pattern that can be caused by either recombination or by convergent amino acid evolution driven by natural selection. We perform a detailed analysis of the synonymous divergence, which is less likely to be affected by selection than amino acid divergence, between human SARS-CoV-2 and related strains. We show that the synonymous divergence between the bat-derived viruses and SARS-CoV-2 is larger than between GD410721 and SARS-CoV-2 in the RBD, providing strong additional support for the recombination hypothesis. However, the synonymous divergence between pangolin strain and SARS-CoV-2 is also relatively high, which is not consistent with a recent recombination between them, instead, it suggests a recombination into RaTG13. We also find a 14-fold increase in the d[SUB]N[/SUB] /d[SUB]S[/SUB] ratio from the lineage leading to SARS-CoV-2 to the strains of the current pandemic, suggesting that the vast majority of nonsynonymous mutations currently segregating within the human strains have a negative impact on viral fitness. Finally, we estimate that the time to the most recent common ancestor of SARS-CoV-2 and RaTG13 or RmYN02 based on synonymous divergence is 51.71 years (95% CI, 28.11-75.31) and 37.02 years (95% CI, 18.19-55.85), respectively.
Keywords: SARS-CoV-2; molecular evolution; synonymous mutations.