tetano
Editor, Senior Moderator
Viruses
. 2022 May 5;14(5):965.
doi: 10.3390/v14050965.
Detection of SARS-CoV-2 Nucleocapsid, Spike, and Neutralizing Antibodies in Vaccinated Japanese
Rie Midorikawa[SUP] 1 [/SUP], Moriyuki Nakama[SUP] 1 2 [/SUP], Hiroshi Furukawa[SUP] 3 4 [/SUP], Shomi Oka[SUP] 3 4 [/SUP], Takashi Higuchi[SUP] 3 5 [/SUP], Hideaki Nagai[SUP] 6 [/SUP], Nobuhiro Nagai[SUP] 1 [/SUP], Shigeto Tohma[SUP] 3 4 [/SUP]
Affiliations
Abstract
Serological detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N), spike (S), and neutralizing antibodies (Abs) is commonly undertaken to evaluate the efficacy of vaccination. However, the relative efficiency of different SARS-CoV-2 Ab detection systems has not been extensively investigated. Here, we evaluated serological test systems in vaccinated Japanese. SARS-CoV-2 N, S, and neutralizing Abs in sera of 375 healthy subjects a mean 253 days after vaccination were assessed. The sensitivity of Elecsys Anti-SARS-CoV-2 S (Roche S) and Anti-SARS-CoV-2 S IgG (Fujirebio S) was 100% and 98.9%, respectively, with a specificity of 100% for both. The sensitivity of Anti-SARS-CoV-2 neutralizing Ab (MBL Neu) was 2.7%, and the specificity was 100%. Fujirebio S correlated with Roche S (rho = 0.9182, p = 3.97 × 10[SUP]-152[/SUP]). Fujirebio S (rho = 0.1295, p = 0.0121) and Roche S (rho = 0.1232, p = 0.0170) correlated weakly with MBL Neu. However, Roche S did correlate with MBL Neu in patients with COVID-19 (rho = 0.8299, p = 1.01 × 10[SUP]-12[/SUP]) and in healthy subjects more recently after vaccination (mean of 90 days, rho = 0.5306, p = 0.0003). Thus, the Fujirebio S and Roche S results were very similar, but neither correlated with neutralizing antibody titers by MBL Neu at a later time after vaccination.
Keywords: SARS-CoV-2 antibody; neutralizing antibody; nucleocapsid; spike; vaccination
. 2022 May 5;14(5):965.
doi: 10.3390/v14050965.
Detection of SARS-CoV-2 Nucleocapsid, Spike, and Neutralizing Antibodies in Vaccinated Japanese
Rie Midorikawa[SUP] 1 [/SUP], Moriyuki Nakama[SUP] 1 2 [/SUP], Hiroshi Furukawa[SUP] 3 4 [/SUP], Shomi Oka[SUP] 3 4 [/SUP], Takashi Higuchi[SUP] 3 5 [/SUP], Hideaki Nagai[SUP] 6 [/SUP], Nobuhiro Nagai[SUP] 1 [/SUP], Shigeto Tohma[SUP] 3 4 [/SUP]
Affiliations
- PMID: 35632710
- DOI: 10.3390/v14050965
Abstract
Serological detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N), spike (S), and neutralizing antibodies (Abs) is commonly undertaken to evaluate the efficacy of vaccination. However, the relative efficiency of different SARS-CoV-2 Ab detection systems has not been extensively investigated. Here, we evaluated serological test systems in vaccinated Japanese. SARS-CoV-2 N, S, and neutralizing Abs in sera of 375 healthy subjects a mean 253 days after vaccination were assessed. The sensitivity of Elecsys Anti-SARS-CoV-2 S (Roche S) and Anti-SARS-CoV-2 S IgG (Fujirebio S) was 100% and 98.9%, respectively, with a specificity of 100% for both. The sensitivity of Anti-SARS-CoV-2 neutralizing Ab (MBL Neu) was 2.7%, and the specificity was 100%. Fujirebio S correlated with Roche S (rho = 0.9182, p = 3.97 × 10[SUP]-152[/SUP]). Fujirebio S (rho = 0.1295, p = 0.0121) and Roche S (rho = 0.1232, p = 0.0170) correlated weakly with MBL Neu. However, Roche S did correlate with MBL Neu in patients with COVID-19 (rho = 0.8299, p = 1.01 × 10[SUP]-12[/SUP]) and in healthy subjects more recently after vaccination (mean of 90 days, rho = 0.5306, p = 0.0003). Thus, the Fujirebio S and Roche S results were very similar, but neither correlated with neutralizing antibody titers by MBL Neu at a later time after vaccination.
Keywords: SARS-CoV-2 antibody; neutralizing antibody; nucleocapsid; spike; vaccination