tetano
Editor, Senior Moderator
Anal Bioanal Chem. 2015 Aug 22. [Epub ahead of print]
[h=1]A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera.[/h] Jarocka U[SUP]1[/SUP], Sawicka R, Stachyra A, G?ra-Sochacka A, Sirko A, Zag?rski-Ostoja W, Sączyńska V, Porębska A, Dehaen W, Radecki J, Radecka H.
[h=3]Author information[/h]
[h=3]Abstract[/h] This paper describes the development of a biosensor for the detection of anti-hemagglutinin antibodies against the influenza virus hemagglutinin. The steps of biosensor fabrications are as follows: (i) creation of a mixed layer containing the thiol derivative of dipyrromethene and 4-mercapto-1-butanol, (ii) complexation of Cu(II) ions, (iii) oriented immobilization of the recombinant histidine-tagged hemagglutinin, and (iv) filling free spaces with bovine serum albumin. The interactions between recombinants hemagglutinin from the highly pathogenic avian influenza virus type H5N1 and anti-hemagglutinin H5 monoclonal antibodies were explored with Osteryoung square-wave voltammetry. The biosensor displayed a good detection limit of 2.4 pg/mL, quantification limit of 7.2 pg/mL, and dynamic range from 4.0 to 100.0 pg/mL in buffer. In addition, this analytical device was applied for the detection of antibodies in hen sera from individuals vaccinated and non-vaccinated against the avian influenza virus type H5N1. The limit of detection for the assay was the dilution of sera 1: 7 ? 10[SUP]6[/SUP], which is about 200 times better than the enzyme-linked immunosorbent assay. Graphical Abstract Scheme of immunosensor based on dipyrromethene-Cu(II)-Histidine-tagged hemagglutinin deposited on gold electrode.
PMID: 26297459 [PubMed - as supplied by publisher]
[h=1]A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera.[/h] Jarocka U[SUP]1[/SUP], Sawicka R, Stachyra A, G?ra-Sochacka A, Sirko A, Zag?rski-Ostoja W, Sączyńska V, Porębska A, Dehaen W, Radecki J, Radecka H.
[h=3]Author information[/h]
[h=3]Abstract[/h] This paper describes the development of a biosensor for the detection of anti-hemagglutinin antibodies against the influenza virus hemagglutinin. The steps of biosensor fabrications are as follows: (i) creation of a mixed layer containing the thiol derivative of dipyrromethene and 4-mercapto-1-butanol, (ii) complexation of Cu(II) ions, (iii) oriented immobilization of the recombinant histidine-tagged hemagglutinin, and (iv) filling free spaces with bovine serum albumin. The interactions between recombinants hemagglutinin from the highly pathogenic avian influenza virus type H5N1 and anti-hemagglutinin H5 monoclonal antibodies were explored with Osteryoung square-wave voltammetry. The biosensor displayed a good detection limit of 2.4 pg/mL, quantification limit of 7.2 pg/mL, and dynamic range from 4.0 to 100.0 pg/mL in buffer. In addition, this analytical device was applied for the detection of antibodies in hen sera from individuals vaccinated and non-vaccinated against the avian influenza virus type H5N1. The limit of detection for the assay was the dilution of sera 1: 7 ? 10[SUP]6[/SUP], which is about 200 times better than the enzyme-linked immunosorbent assay. Graphical Abstract Scheme of immunosensor based on dipyrromethene-Cu(II)-Histidine-tagged hemagglutinin deposited on gold electrode.
PMID: 26297459 [PubMed - as supplied by publisher]