tetano
Editor, Senior Moderator
Acta Naturae. 2016 Apr-Jun;8(2):116-26.
[h=1]A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.[/h] Stepanova LA[SUP]1[/SUP], Sergeeva MV[SUP]1[/SUP], Shuklina MA[SUP]1[/SUP], Shaldzhyan AA[SUP]1[/SUP], Potapchuk MV[SUP]1[/SUP], Korotkov AV[SUP]1[/SUP], Tsybalova LM[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses.
[h=4]KEYWORDS:[/h] HA2; flagellin; influenza; recombinant protein; vaccine
PMID: 27437146
[PubMed]
[h=1]A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.[/h] Stepanova LA[SUP]1[/SUP], Sergeeva MV[SUP]1[/SUP], Shuklina MA[SUP]1[/SUP], Shaldzhyan AA[SUP]1[/SUP], Potapchuk MV[SUP]1[/SUP], Korotkov AV[SUP]1[/SUP], Tsybalova LM[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses.
[h=4]KEYWORDS:[/h] HA2; flagellin; influenza; recombinant protein; vaccine
PMID: 27437146
[PubMed]