tetano
Editor, Senior Moderator
J Formos Med Assoc. 2012 Dec;111(12):693-7. doi: 10.1016/j.jfma.2011.11.029. Epub 2012 May 9.
A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections.
Lu CY, Chang LY, Chen PJ, Xia NS, Shao PL, Huang LM.
Source
Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
Abstract
BACKGROUND/PURPOSE:
Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples.
METHODS:
A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested.
RESULTS:
Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively.
CONCLUSION:
The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity.
Copyright ? 2012. Published by Elsevier B.V.
PMID:
23265748
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23265748
A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections.
Lu CY, Chang LY, Chen PJ, Xia NS, Shao PL, Huang LM.
Source
Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
Abstract
BACKGROUND/PURPOSE:
Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples.
METHODS:
A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested.
RESULTS:
Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively.
CONCLUSION:
The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity.
Copyright ? 2012. Published by Elsevier B.V.
PMID:
23265748
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23265748