tetano
Editor, Senior Moderator
Appl Microbiol Biotechnol. 2020 Jan 2. doi: 10.1007/s00253-019-10217-2. [Epub ahead of print] [h=1]A new abyssomicin polyketide with anti-influenza A virus activity from a marine-derived Verrucosispora sp. MS100137.[/h]
Zhang J[SUP]1[/SUP], Li B[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Qin Y[SUP]3[/SUP], Karthik L[SUP]1,[/SUP][SUP]4[/SUP], Zhu G[SUP]1[/SUP], Hou C[SUP]1[/SUP], Jiang L[SUP]1[/SUP], Liu M[SUP]5[/SUP], Ye X[SUP]3[/SUP], Liu M[SUP]3[/SUP], Hsiang T[SUP]6[/SUP], Dai H[SUP]7[/SUP], Zhang L[SUP]8[/SUP], Liu X[SUP]9[/SUP].
[h=3]Author information[/h] 1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. 2 Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601, Anhui Province, China. 3 Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. 4 Sri Shakthi Institute of Engineering and Technology (Autonomous), Coimbatore, Tamil Nadu, India. 5 Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, 4111, Australia. 6 School of Environmental Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. 7 Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. daihq@im.ac.cn. 8 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. lxzhang@ecust.edu.cn. 9 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. liuxueting@ecust.edu.cn.
[h=3]Abstract[/h] Marine microorganisms live in dramatically different environments and have attracted much attention for their structurally unique natural products with potential strong biological activity. Based on the one strain-many compounds (OSMAC) strategy and liquid chromatography mass spectrometry (LC-MS) methods, our continuing efforts on the investigation of novel active compounds from marine Verrucosispora sp. MS100137 has led to the identification of a new polycyclic metabolite, abyssomicin Y (1), together with six known abyssomicin and proximicin analogs (2-7). Abyssomicin Y is a type I abyssomicin with an epoxide group at C-8 and C-9. Compounds 1-3 showed potent inhibitory effects against the influenza A virus; their observed inhibition rates were 97.9%, 98.3%, and 95.9%, respectively, at a concentration of 10 μM, and they displayed lower cytotoxicity than 4. The structures were determined by different NMR techniques and HRMS experiments. This investigation revealed that OSMAC could serve as a useful method for enabling the activation of the silent genes in the microorganism and for the formation of previously unreported active secondary metabolites.
[h=4]KEYWORDS:[/h] Abyssomicin; Anti-influenza A virus; Marine-derived Verrucosispora sp.; OSMAC; Proximicin
PMID: 31894364 DOI: 10.1007/s00253-019-10217-2
Zhang J[SUP]1[/SUP], Li B[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Qin Y[SUP]3[/SUP], Karthik L[SUP]1,[/SUP][SUP]4[/SUP], Zhu G[SUP]1[/SUP], Hou C[SUP]1[/SUP], Jiang L[SUP]1[/SUP], Liu M[SUP]5[/SUP], Ye X[SUP]3[/SUP], Liu M[SUP]3[/SUP], Hsiang T[SUP]6[/SUP], Dai H[SUP]7[/SUP], Zhang L[SUP]8[/SUP], Liu X[SUP]9[/SUP].
[h=3]Author information[/h] 1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. 2 Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601, Anhui Province, China. 3 Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. 4 Sri Shakthi Institute of Engineering and Technology (Autonomous), Coimbatore, Tamil Nadu, India. 5 Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, 4111, Australia. 6 School of Environmental Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. 7 Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. daihq@im.ac.cn. 8 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. lxzhang@ecust.edu.cn. 9 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. liuxueting@ecust.edu.cn.
[h=3]Abstract[/h] Marine microorganisms live in dramatically different environments and have attracted much attention for their structurally unique natural products with potential strong biological activity. Based on the one strain-many compounds (OSMAC) strategy and liquid chromatography mass spectrometry (LC-MS) methods, our continuing efforts on the investigation of novel active compounds from marine Verrucosispora sp. MS100137 has led to the identification of a new polycyclic metabolite, abyssomicin Y (1), together with six known abyssomicin and proximicin analogs (2-7). Abyssomicin Y is a type I abyssomicin with an epoxide group at C-8 and C-9. Compounds 1-3 showed potent inhibitory effects against the influenza A virus; their observed inhibition rates were 97.9%, 98.3%, and 95.9%, respectively, at a concentration of 10 μM, and they displayed lower cytotoxicity than 4. The structures were determined by different NMR techniques and HRMS experiments. This investigation revealed that OSMAC could serve as a useful method for enabling the activation of the silent genes in the microorganism and for the formation of previously unreported active secondary metabolites.
[h=4]KEYWORDS:[/h] Abyssomicin; Anti-influenza A virus; Marine-derived Verrucosispora sp.; OSMAC; Proximicin
PMID: 31894364 DOI: 10.1007/s00253-019-10217-2