tetano
Editor, Senior Moderator
Vaccine. 2018 Apr 26. pii: S0264-410X(18)30551-6. doi: 10.1016/j.vaccine.2018.04.054. [Epub ahead of print]
[h=1]A novel adjuvant G3 induces both Th1 and Th2 related immune responses in mice after immunization with a trivalent inactivated split-virion influenza vaccine.[/h] Hjertner B[SUP]1[/SUP], Bengtsson T[SUP]2[/SUP], Morein B[SUP]3[/SUP], Paulie S[SUP]4[/SUP], Fossum C[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A preferred adjuvant should promote both Th1 and Th2 responses. However, most adjuvants in common use are biased towards a Th2-driven response. Therefore, the ability of a novel saponin-based adjuvant G3 to inducing balanced Th1 and Th2 responses in BALB/c mice immunized with a split trivalent seasonal influenza vaccine was evaluated in comparison to that of the adjuvant Al(OH)[SUB]3[/SUB]. Clear differences in the IgG profiles induced by G3, Al(OH)[SUB]3[/SUB] or non-adjuvanted vaccine were recorded. Both adjuvants enhanced high and similar levels of the Th2 associated IgG1 subtype compared to mice given vaccine alone. Only G3 enhanced the IgG2a subclass reflecting a Th1 response, whereas Al(OH)[SUB]3[/SUB] even abrogated the IgG2a production. Accordingly, G3 enhanced the production of IL-2 and IFN-γ and also of IL-2/IFN-γ double secreting cells, emphasizing the strong Th1 driving effect of G3. Only Al(OH)[SUB]3[/SUB] increased splenocyte production of IL-17. Taken together, the results indicate a strong propensity for G3 to induce both Th1 and Th2 driven immune responses.
[h=4]KEYWORDS:[/h] Adjuvant; Cytokine production; Influenza; Th1/Th2
PMID: 29706292 DOI: 10.1016/j.vaccine.2018.04.054
[h=1]A novel adjuvant G3 induces both Th1 and Th2 related immune responses in mice after immunization with a trivalent inactivated split-virion influenza vaccine.[/h] Hjertner B[SUP]1[/SUP], Bengtsson T[SUP]2[/SUP], Morein B[SUP]3[/SUP], Paulie S[SUP]4[/SUP], Fossum C[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A preferred adjuvant should promote both Th1 and Th2 responses. However, most adjuvants in common use are biased towards a Th2-driven response. Therefore, the ability of a novel saponin-based adjuvant G3 to inducing balanced Th1 and Th2 responses in BALB/c mice immunized with a split trivalent seasonal influenza vaccine was evaluated in comparison to that of the adjuvant Al(OH)[SUB]3[/SUB]. Clear differences in the IgG profiles induced by G3, Al(OH)[SUB]3[/SUB] or non-adjuvanted vaccine were recorded. Both adjuvants enhanced high and similar levels of the Th2 associated IgG1 subtype compared to mice given vaccine alone. Only G3 enhanced the IgG2a subclass reflecting a Th1 response, whereas Al(OH)[SUB]3[/SUB] even abrogated the IgG2a production. Accordingly, G3 enhanced the production of IL-2 and IFN-γ and also of IL-2/IFN-γ double secreting cells, emphasizing the strong Th1 driving effect of G3. Only Al(OH)[SUB]3[/SUB] increased splenocyte production of IL-17. Taken together, the results indicate a strong propensity for G3 to induce both Th1 and Th2 driven immune responses.
[h=4]KEYWORDS:[/h] Adjuvant; Cytokine production; Influenza; Th1/Th2
PMID: 29706292 DOI: 10.1016/j.vaccine.2018.04.054