tetano
Editor, Senior Moderator
J Vet Sci. 2016 Mar;17(1):71-8. doi: 10.4142/jvs.2016.17.1.71. Epub 2016 Mar 22.
[h=1]A novel M2e-multiple antigenic peptide providing heterologous protection in mice.[/h] Wen F[SUP]1[/SUP], Ma JH[SUP]1[/SUP], Yu H[SUP]1[/SUP], Yang FR[SUP]1[/SUP], Huang M[SUP]1[/SUP], Zhou YJ[SUP]1[/SUP], Li ZJ[SUP]1[/SUP], Wang XH[SUP]1[/SUP], Li GX[SUP]1[/SUP], Jiang YF[SUP]1[/SUP], Tong W[SUP]1[/SUP], Tong GZ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
[h=4]KEYWORDS:[/h] H3N2; M2e-multiple antigenic peptide; high-yield; inactivated vaccine; swine influenza virus
PMID: 27051342 [PubMed - in process] PMCID: PMC4808646 Free PMC Article
[h=1]A novel M2e-multiple antigenic peptide providing heterologous protection in mice.[/h] Wen F[SUP]1[/SUP], Ma JH[SUP]1[/SUP], Yu H[SUP]1[/SUP], Yang FR[SUP]1[/SUP], Huang M[SUP]1[/SUP], Zhou YJ[SUP]1[/SUP], Li ZJ[SUP]1[/SUP], Wang XH[SUP]1[/SUP], Li GX[SUP]1[/SUP], Jiang YF[SUP]1[/SUP], Tong W[SUP]1[/SUP], Tong GZ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
[h=4]KEYWORDS:[/h] H3N2; M2e-multiple antigenic peptide; high-yield; inactivated vaccine; swine influenza virus
PMID: 27051342 [PubMed - in process] PMCID: PMC4808646 Free PMC Article