tetano
Editor, Senior Moderator
J Clin Virol. 2017 Sep 1;96:12-16. doi: 10.1016/j.jcv.2017.08.014. [Epub ahead of print]
[h=1]A novel real-time RT-PCR assay for influenza C tested in Peruvian children.[/h] Howard LM[SUP]1[/SUP], Johnson M[SUP]2[/SUP], Gil AI[SUP]3[/SUP], Pekosz A[SUP]4[/SUP], Griffin MR[SUP]5[/SUP], Edwards KM[SUP]1[/SUP], Lanata CF[SUP]6[/SUP], Grijalva CG[SUP]5[/SUP], Williams JV[SUP]7[/SUP]; RESPIRA-PERU Group.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.
[h=4]OBJECTIVES:[/h] To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.
[h=4]STUDY DESIGN:[/h] Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences. Primers and probes were designed based on conserved regions. Multiple primer-probe sets were tested against multiple ICV strains.
[h=4]RESULTS:[/h] The ICV M and NP genes offered the most conserved sequence regions. Primers and probes based on newer sequence data offered enhanced detection of ICV, especially for low titer specimens. An NP-targeted assay yielded the best performance and was capable of detecting 10-100 RNA copies per reaction. The NP assay detected multiple clinical isolates of ICV collected in a field epidemiology study conducted in Peru.
[h=4]CONCLUSIONS:[/h] We report a new real-time RT-PCR assay for ICV with high sensitivity and specificity.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Diagnostics; Influenza C; Real-time RT-PCR
PMID: 28917132 DOI: 10.1016/j.jcv.2017.08.014
[h=1]A novel real-time RT-PCR assay for influenza C tested in Peruvian children.[/h] Howard LM[SUP]1[/SUP], Johnson M[SUP]2[/SUP], Gil AI[SUP]3[/SUP], Pekosz A[SUP]4[/SUP], Griffin MR[SUP]5[/SUP], Edwards KM[SUP]1[/SUP], Lanata CF[SUP]6[/SUP], Grijalva CG[SUP]5[/SUP], Williams JV[SUP]7[/SUP]; RESPIRA-PERU Group.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.
[h=4]OBJECTIVES:[/h] To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.
[h=4]STUDY DESIGN:[/h] Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences. Primers and probes were designed based on conserved regions. Multiple primer-probe sets were tested against multiple ICV strains.
[h=4]RESULTS:[/h] The ICV M and NP genes offered the most conserved sequence regions. Primers and probes based on newer sequence data offered enhanced detection of ICV, especially for low titer specimens. An NP-targeted assay yielded the best performance and was capable of detecting 10-100 RNA copies per reaction. The NP assay detected multiple clinical isolates of ICV collected in a field epidemiology study conducted in Peru.
[h=4]CONCLUSIONS:[/h] We report a new real-time RT-PCR assay for ICV with high sensitivity and specificity.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Diagnostics; Influenza C; Real-time RT-PCR
PMID: 28917132 DOI: 10.1016/j.jcv.2017.08.014