tetano
Editor, Senior Moderator
Sensors (Basel). 2017 Oct 16;17(10). pii: E2363. doi: 10.3390/s17102363.
[h=1]A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection.[/h] Wong CL[SUP]1[/SUP], Chua M[SUP]2[/SUP], Mittman H[SUP]3[/SUP], Choo LX[SUP]4[/SUP], Lim HQ[SUP]5[/SUP], Olivo M[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 ? 10[SUP]-6[/SUP] refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL[SUP]-1[/SUP], compared to 1 μg mL[SUP]-1[/SUP] and 0.5 μg mL[SUP]-1[/SUP], as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection.
[h=4]KEYWORDS:[/h] H5N1; SPR biosensor; avian influenza A; phase-intensity detection; surface plasmon resonance
PMID: 29035344 DOI: 10.3390/s17102363
[h=1]A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection.[/h] Wong CL[SUP]1[/SUP], Chua M[SUP]2[/SUP], Mittman H[SUP]3[/SUP], Choo LX[SUP]4[/SUP], Lim HQ[SUP]5[/SUP], Olivo M[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 ? 10[SUP]-6[/SUP] refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL[SUP]-1[/SUP], compared to 1 μg mL[SUP]-1[/SUP] and 0.5 μg mL[SUP]-1[/SUP], as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection.
[h=4]KEYWORDS:[/h] H5N1; SPR biosensor; avian influenza A; phase-intensity detection; surface plasmon resonance
PMID: 29035344 DOI: 10.3390/s17102363