tetano
Editor, Senior Moderator
J Med Virol. 2018 Dec 29. doi: 10.1002/jmv.25387. [Epub ahead of print]
[h=1]A Sandwich ELISA for Detecting the Hemagglutinin of Avian Influenza A (H10N8) Virus.[/h] Chen L[SUP]1,[/SUP][SUP]2[/SUP], Ruan F[SUP]1,[/SUP][SUP]2[/SUP], Liu M[SUP]3,[/SUP][SUP]4[/SUP], Zhou J[SUP]2[/SUP], Song W[SUP]5[/SUP], Qin K[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Novel influenza A virus (H10N8) infected human with fatality in China during 2013-2014. It is important to detect such non-prevalent subtype influenza A virus in clinic and regular surveillance in the early stage for effective control and prevention from the potential pandemic. Unavailability of convenient rapid diagnosis for this subtype virus in resources-limited setting is an obstacle for timely recognizing human case. In the present study, a panel of mouse H10 specific monoclonal antibodies (mAbs) was generated, two of which were used to develop a sandwich ELISA for detecting the hemagglutinin of avian influenza A (H10N8) virus. ELISA results showed high sensitivity with the lowest detection limit of 0.5HAU/50μl for live virus, which laid a foundation for clinic use as a promising diagnostic methodology. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] H10 subtype; hemagglutinin; monoclonal antibody; sandwich ELISA
PMID: 30593681 DOI: 10.1002/jmv.25387
[h=1]A Sandwich ELISA for Detecting the Hemagglutinin of Avian Influenza A (H10N8) Virus.[/h] Chen L[SUP]1,[/SUP][SUP]2[/SUP], Ruan F[SUP]1,[/SUP][SUP]2[/SUP], Liu M[SUP]3,[/SUP][SUP]4[/SUP], Zhou J[SUP]2[/SUP], Song W[SUP]5[/SUP], Qin K[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Novel influenza A virus (H10N8) infected human with fatality in China during 2013-2014. It is important to detect such non-prevalent subtype influenza A virus in clinic and regular surveillance in the early stage for effective control and prevention from the potential pandemic. Unavailability of convenient rapid diagnosis for this subtype virus in resources-limited setting is an obstacle for timely recognizing human case. In the present study, a panel of mouse H10 specific monoclonal antibodies (mAbs) was generated, two of which were used to develop a sandwich ELISA for detecting the hemagglutinin of avian influenza A (H10N8) virus. ELISA results showed high sensitivity with the lowest detection limit of 0.5HAU/50μl for live virus, which laid a foundation for clinic use as a promising diagnostic methodology. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] H10 subtype; hemagglutinin; monoclonal antibody; sandwich ELISA
PMID: 30593681 DOI: 10.1002/jmv.25387