tetano
Editor, Senior Moderator
Arch Virol. 2019 Jun 21. doi: 10.1007/s00705-019-04328-4. [Epub ahead of print]
[h=1]A single amino acid change in hemagglutinin reduces the cross-reactivity of antiserum against an equine influenza vaccine strain.[/h] Nemoto M[SUP]1[/SUP], Yamayoshi S[SUP]2[/SUP], Bannai H[SUP]3[/SUP], Tsujimura K[SUP]3[/SUP], Kokado H[SUP]3[/SUP], Kawaoka Y[SUP]2,[/SUP][SUP]4[/SUP], Yamanaka T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Equine influenza virus is an important pathogen for the horse industry because of its economic impact, and vaccination is a key control measure. Our previous work suggested that a mutation at position 144 in the hemagglutinin of Florida sublineage clade 2 viruses reduces the cross-neutralizing activity of antiserum against a former vaccine strain. To confirm this suggestion, here, we generated viruses by reverse genetics. Antibody titers against the mutated viruses were one-tenth to one-sixteenth of those against the former vaccine strain. Our findings confirm that this single amino acid substitution reduces the cross-reactivity of antiserum against this former Japanese vaccine.
PMID: 31227892 DOI: 10.1007/s00705-019-04328-4
[h=1]A single amino acid change in hemagglutinin reduces the cross-reactivity of antiserum against an equine influenza vaccine strain.[/h] Nemoto M[SUP]1[/SUP], Yamayoshi S[SUP]2[/SUP], Bannai H[SUP]3[/SUP], Tsujimura K[SUP]3[/SUP], Kokado H[SUP]3[/SUP], Kawaoka Y[SUP]2,[/SUP][SUP]4[/SUP], Yamanaka T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Equine influenza virus is an important pathogen for the horse industry because of its economic impact, and vaccination is a key control measure. Our previous work suggested that a mutation at position 144 in the hemagglutinin of Florida sublineage clade 2 viruses reduces the cross-neutralizing activity of antiserum against a former vaccine strain. To confirm this suggestion, here, we generated viruses by reverse genetics. Antibody titers against the mutated viruses were one-tenth to one-sixteenth of those against the former vaccine strain. Our findings confirm that this single amino acid substitution reduces the cross-reactivity of antiserum against this former Japanese vaccine.
PMID: 31227892 DOI: 10.1007/s00705-019-04328-4