tetano
Editor, Senior Moderator
ACS Appl Bio Mater
. 2025 Aug 21.
doi: 10.1021/acsabm.5c01361. Online ahead of print. Needle-Free Transdermal Patch for Influenza Vaccination
Keisuke Tanaka[SUP] 1 [/SUP], Hazuki Masaki[SUP] 1 [/SUP], Kosuke Minamihata[SUP] 1 [/SUP], Rie Wakabayashi[SUP] 1 [/SUP], Yoshirou Kawaguchi[SUP] 1 [/SUP], Noriho Kamiya[SUP] 1 [/SUP], Masahiro Goto[SUP] 1 [/SUP]
Affiliations
Transdermal vaccination offers a promising alternative to conventional injections by eliminating needle-related risks and improving patient satisfaction. However, because the stratum corneum poses a considerable barrier to antigen delivery, innovative approaches for effective transdermal immunization are necessary. We propose the transdermal influenza vaccine by using a solid-in-oil (S/O) dispersion-based patch formulation for the transdermal delivery of hemagglutinin antigen and CpG oligonucleotide adjuvant. The formulation was prepared by incorporating a S/O dispersion into an adhesive matrix to enhance antigen skin permeation. In vitro and in vivo skin permeation studies demonstrated that the S/O patch achieved 2.5-fold greater antigen delivery compared with a S/O dispersion. Immunization studies in mice revealed that the S/O patch induced robust IgG antibody titers that were comparable to subcutaneous injection, highlighting the potential of the patch as a viable transdermal vaccine platform. Furthermore, histological analysis indicated there was minimal skin irritation, with transient effects resolving within 72 h. These findings suggest that the S/O patch formulation can enable efficient, noninvasive transdermal immunization, offering a practical alternative to injection-based influenza vaccines.
Keywords: drug delivery system; immune response; influenza vaccine; skin permeation; transdermal delivery.
. 2025 Aug 21.
doi: 10.1021/acsabm.5c01361. Online ahead of print. Needle-Free Transdermal Patch for Influenza Vaccination
Keisuke Tanaka[SUP] 1 [/SUP], Hazuki Masaki[SUP] 1 [/SUP], Kosuke Minamihata[SUP] 1 [/SUP], Rie Wakabayashi[SUP] 1 [/SUP], Yoshirou Kawaguchi[SUP] 1 [/SUP], Noriho Kamiya[SUP] 1 [/SUP], Masahiro Goto[SUP] 1 [/SUP]
Affiliations
- PMID: 40839797
- DOI: 10.1021/acsabm.5c01361
Transdermal vaccination offers a promising alternative to conventional injections by eliminating needle-related risks and improving patient satisfaction. However, because the stratum corneum poses a considerable barrier to antigen delivery, innovative approaches for effective transdermal immunization are necessary. We propose the transdermal influenza vaccine by using a solid-in-oil (S/O) dispersion-based patch formulation for the transdermal delivery of hemagglutinin antigen and CpG oligonucleotide adjuvant. The formulation was prepared by incorporating a S/O dispersion into an adhesive matrix to enhance antigen skin permeation. In vitro and in vivo skin permeation studies demonstrated that the S/O patch achieved 2.5-fold greater antigen delivery compared with a S/O dispersion. Immunization studies in mice revealed that the S/O patch induced robust IgG antibody titers that were comparable to subcutaneous injection, highlighting the potential of the patch as a viable transdermal vaccine platform. Furthermore, histological analysis indicated there was minimal skin irritation, with transient effects resolving within 72 h. These findings suggest that the S/O patch formulation can enable efficient, noninvasive transdermal immunization, offering a practical alternative to injection-based influenza vaccines.
Keywords: drug delivery system; immune response; influenza vaccine; skin permeation; transdermal delivery.