tetano
Editor, Senior Moderator
ACS Cent Sci
. 2020 Dec 23;6(12):2238-2249.
doi: 10.1021/acscentsci.0c00742. Epub 2020 Oct 21.
SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution
Hongye Wang[SUP] 1 [/SUP], Xian Wu[SUP] 2 [/SUP], Xiaomei Zhang[SUP] 1 [/SUP], Xin Hou[SUP] 2 [/SUP], Te Liang[SUP] 1 [/SUP], Dan Wang[SUP] 1 [/SUP], Fei Teng[SUP] 3 [/SUP], Jiayu Dai[SUP] 1 [/SUP], Hu Duan[SUP] 1 [/SUP], Shubin Guo[SUP] 3 [/SUP], Yongzhe Li[SUP] 2 [/SUP], Xiaobo Yu[SUP] 1 [/SUP]
Affiliations
Abstract
Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and in developing diagnostic tests and vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray to analyze antibody interactions at the amino acid resolution. With the array, we demonstrate the feasibility of employing SARS-CoV-1 antibodies to detect the SARS-CoV-2 nucleocapsid phosphoprotein. The first landscape of B-cell epitopes for SARS-CoV-2 IgM and IgG antibodies in the serum of 10 coronavirus disease of 2019 (COVID-19) patients with early infection is also constructed. With array data and structural analysis, a peptide epitope for neutralizing antibodies within the SARS-CoV-2 spike receptor-binding domain's interaction interface with the angiotensin-converting enzyme 2 receptor was predicted. All the results demonstrate the utility of our microarray as a platform to determine the changes of antibody responses in COVID-19 patients and animal models as well as to identify potential targets for diagnosis and treatment.
. 2020 Dec 23;6(12):2238-2249.
doi: 10.1021/acscentsci.0c00742. Epub 2020 Oct 21.
SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution
Hongye Wang[SUP] 1 [/SUP], Xian Wu[SUP] 2 [/SUP], Xiaomei Zhang[SUP] 1 [/SUP], Xin Hou[SUP] 2 [/SUP], Te Liang[SUP] 1 [/SUP], Dan Wang[SUP] 1 [/SUP], Fei Teng[SUP] 3 [/SUP], Jiayu Dai[SUP] 1 [/SUP], Hu Duan[SUP] 1 [/SUP], Shubin Guo[SUP] 3 [/SUP], Yongzhe Li[SUP] 2 [/SUP], Xiaobo Yu[SUP] 1 [/SUP]
Affiliations
- PMID: 33372199
- PMCID: PMC7586461
- DOI: 10.1021/acscentsci.0c00742
Abstract
Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and in developing diagnostic tests and vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray to analyze antibody interactions at the amino acid resolution. With the array, we demonstrate the feasibility of employing SARS-CoV-1 antibodies to detect the SARS-CoV-2 nucleocapsid phosphoprotein. The first landscape of B-cell epitopes for SARS-CoV-2 IgM and IgG antibodies in the serum of 10 coronavirus disease of 2019 (COVID-19) patients with early infection is also constructed. With array data and structural analysis, a peptide epitope for neutralizing antibodies within the SARS-CoV-2 spike receptor-binding domain's interaction interface with the angiotensin-converting enzyme 2 receptor was predicted. All the results demonstrate the utility of our microarray as a platform to determine the changes of antibody responses in COVID-19 patients and animal models as well as to identify potential targets for diagnosis and treatment.