tetano
Editor, Senior Moderator
Virology. 2014 Dec 24;476C:141-150. doi: 10.1016/j.virol.2014.12.016. [Epub ahead of print]
[h=1]Actin-myosin network is required for proper assembly of influenza virus particles.[/h] Kumakura M[SUP]1[/SUP], Kawaguchi A[SUP]2[/SUP], Nagata K[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation.
Copyright ? 2014 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Blebbistatin; Influenza virus assembly; Non-muscle myosin II
PMID: 25543965 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25543965
[h=1]Actin-myosin network is required for proper assembly of influenza virus particles.[/h] Kumakura M[SUP]1[/SUP], Kawaguchi A[SUP]2[/SUP], Nagata K[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation.
Copyright ? 2014 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Blebbistatin; Influenza virus assembly; Non-muscle myosin II
PMID: 25543965 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25543965