tetano
Editor, Senior Moderator
Immunol Lett. 2010 Aug 12. [Epub ahead of print]
Adjuvant effect of Bacillus firmus on the expression of cytokines and toll-like receptors in mouse nasopharynx-associated lymphoid tissue (NALT) after intranasal immunization with inactivated influenza virus type A.
Peter Z, Ale? T, Martina H, Olga N, Marie J, Katar?na K, Dana C, Jaroslav J, Ivan S, Proke?ov? L.
Charles University in Prague, First Faculty of Medicine, Institute of Immunology and Microbiology, Studnickova 7, 128 00 Prague, Czech Republic.
Abstract
Due to the persisting threat of development of new highly pathogenic influenza A subtypes, a mucosal vaccination which would induce a potent and cross-protective reaction is desirable. We succeeded in mucosal immunization of mice with an inactivated influenza A virus by using delipidated Bacillus firmus (DBF) as adjuvant. The mechanism of adjuvant effect was followed in NALT by comparing the response after intranasal immunization by inactivated influenza virus type A (H1N1) alone, adjuvant alone (DBF), or by a mixture of virus+DBF. Expression of selected gene groups was tested via qPCR at 7 different time points: cytokines (IL-2, IFN-gamma, IL-4, IL-6, & IL-10), type I interferons (IFN-alpha4, IFN-alpha11, IFN-alpha12, & IFN-beta), toll-like receptors (TLR2, TLR3, TLR7, & TLR9), iNOS and CCR7. Intranasally administered DBF and the mixture of virus+DBF induced an elevated expression of IFN-gamma, IL-6 and IL-10 cytokines, type I interferons, iNOS, and pDC markers in NALT. Multimarker qPCR data was analyzed by relative quantification and by principal component analysis. DBF has been shown to be a very efficient adjuvant for the stimulation of innate immunity after IN immunization. DBF accelerated, increased, and prolonged the antiviral response.
PMID: 20709105 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20709105
Adjuvant effect of Bacillus firmus on the expression of cytokines and toll-like receptors in mouse nasopharynx-associated lymphoid tissue (NALT) after intranasal immunization with inactivated influenza virus type A.
Peter Z, Ale? T, Martina H, Olga N, Marie J, Katar?na K, Dana C, Jaroslav J, Ivan S, Proke?ov? L.
Charles University in Prague, First Faculty of Medicine, Institute of Immunology and Microbiology, Studnickova 7, 128 00 Prague, Czech Republic.
Abstract
Due to the persisting threat of development of new highly pathogenic influenza A subtypes, a mucosal vaccination which would induce a potent and cross-protective reaction is desirable. We succeeded in mucosal immunization of mice with an inactivated influenza A virus by using delipidated Bacillus firmus (DBF) as adjuvant. The mechanism of adjuvant effect was followed in NALT by comparing the response after intranasal immunization by inactivated influenza virus type A (H1N1) alone, adjuvant alone (DBF), or by a mixture of virus+DBF. Expression of selected gene groups was tested via qPCR at 7 different time points: cytokines (IL-2, IFN-gamma, IL-4, IL-6, & IL-10), type I interferons (IFN-alpha4, IFN-alpha11, IFN-alpha12, & IFN-beta), toll-like receptors (TLR2, TLR3, TLR7, & TLR9), iNOS and CCR7. Intranasally administered DBF and the mixture of virus+DBF induced an elevated expression of IFN-gamma, IL-6 and IL-10 cytokines, type I interferons, iNOS, and pDC markers in NALT. Multimarker qPCR data was analyzed by relative quantification and by principal component analysis. DBF has been shown to be a very efficient adjuvant for the stimulation of innate immunity after IN immunization. DBF accelerated, increased, and prolonged the antiviral response.
PMID: 20709105 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20709105