tetano
Editor, Senior Moderator
Adv Compos Hybrid Mater
. 2023;6(1):49.
doi: 10.1007/s42114-023-00630-7. Epub 2023 Jan 26.
Ultrasensitive electrochemical biosensors based on zinc sulfide/graphene hybrid for rapid detection of SARS-CoV-2
Shatila Sarwar[SUP] 1 [/SUP], Mao-Chia Lin[SUP] 1 [/SUP], Carolina Amezaga[SUP] 2 [/SUP], Zhen Wei[SUP] 3 [/SUP], Etinosa Iyayi[SUP] 4 [/SUP], Haseena Polk[SUP] 4 [/SUP], Ruigang Wang[SUP] 3 [/SUP], Honghe Wang[SUP] 4 [/SUP], Xinyu Zhang[SUP] 1 [/SUP]
Affiliations
Abstract
The coronavirus disease 2019 (COVID-19) is a highly contagious and fatal disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In general, the diagnostic tests for COVID-19 are based on the detection of nucleic acid, antibodies, and protein. Among different analytes, the gold standard of the COVID-19 test is the viral nucleic acid detection performed by the quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. However, the gold standard test is time-consuming and requires expensive instrumentation, as well as trained personnel. Herein, we report an ultrasensitive electrochemical biosensor based on zinc sulfide/graphene (ZnS/graphene) nanocomposite for rapid and direct nucleic acid detection of SARS-CoV-2. We demonstrated a simple one-step route for manufacturing ZnS/graphene by employing an ultrafast (90 s) microwave-based non-equilibrium heating approach. The biosensor assay involves the hybridization of target DNA or RNA samples with probes that are immersed into a redox active electrolyte, which are detectable by electrochemical measurements. In this study, we have performed the tests for synthetic DNA samples and, SARS-CoV-2 standard samples. Experimental results revealed that the proposed biosensor could detect low concentrations of all different SARS-CoV-2 samples, using such as S, ORF 1a, and ORF 1b gene sequences as targets. This microwave-synthesized ZnS/graphene-based biosensor could be reliably used as an on-site, real-time, and rapid diagnostic test for COVID-19.
Supplementary information: The online version contains supplementary material available at 10.1007/s42114-023-00630-7.
Keywords: COVID-19; DNA biosensor; Graphene; Microwave; Nanocomposite; Zinc sulfide.
. 2023;6(1):49.
doi: 10.1007/s42114-023-00630-7. Epub 2023 Jan 26.
Ultrasensitive electrochemical biosensors based on zinc sulfide/graphene hybrid for rapid detection of SARS-CoV-2
Shatila Sarwar[SUP] 1 [/SUP], Mao-Chia Lin[SUP] 1 [/SUP], Carolina Amezaga[SUP] 2 [/SUP], Zhen Wei[SUP] 3 [/SUP], Etinosa Iyayi[SUP] 4 [/SUP], Haseena Polk[SUP] 4 [/SUP], Ruigang Wang[SUP] 3 [/SUP], Honghe Wang[SUP] 4 [/SUP], Xinyu Zhang[SUP] 1 [/SUP]
Affiliations
- PMID: 36718472
- PMCID: PMC9879254
- DOI: 10.1007/s42114-023-00630-7
Abstract
The coronavirus disease 2019 (COVID-19) is a highly contagious and fatal disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In general, the diagnostic tests for COVID-19 are based on the detection of nucleic acid, antibodies, and protein. Among different analytes, the gold standard of the COVID-19 test is the viral nucleic acid detection performed by the quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. However, the gold standard test is time-consuming and requires expensive instrumentation, as well as trained personnel. Herein, we report an ultrasensitive electrochemical biosensor based on zinc sulfide/graphene (ZnS/graphene) nanocomposite for rapid and direct nucleic acid detection of SARS-CoV-2. We demonstrated a simple one-step route for manufacturing ZnS/graphene by employing an ultrafast (90 s) microwave-based non-equilibrium heating approach. The biosensor assay involves the hybridization of target DNA or RNA samples with probes that are immersed into a redox active electrolyte, which are detectable by electrochemical measurements. In this study, we have performed the tests for synthetic DNA samples and, SARS-CoV-2 standard samples. Experimental results revealed that the proposed biosensor could detect low concentrations of all different SARS-CoV-2 samples, using such as S, ORF 1a, and ORF 1b gene sequences as targets. This microwave-synthesized ZnS/graphene-based biosensor could be reliably used as an on-site, real-time, and rapid diagnostic test for COVID-19.
Supplementary information: The online version contains supplementary material available at 10.1007/s42114-023-00630-7.
Keywords: COVID-19; DNA biosensor; Graphene; Microwave; Nanocomposite; Zinc sulfide.