tetano
Editor, Senior Moderator
Adv Sci (Weinh)
. 2021 Dec 31;e2104192.
doi: 10.1002/advs.202104192. Online ahead of print.
Establishment of Human Pluripotent Stem Cell-Derived Skin Organoids Enabled Pathophysiological Model of SARS-CoV-2 Infection
Jie Ma[SUP] 1 [/SUP], Jia Liu[SUP] 2 [/SUP], Dunqin Gao[SUP] 3 [/SUP], Xiao Li[SUP] 1 [/SUP], Qiyu Zhang[SUP] 4 [/SUP], Luye Lv[SUP] 5 [/SUP], Yujie Wang[SUP] 3 [/SUP], Jun Li[SUP] 4 [/SUP], Yunping Zhu[SUP] 1 6 [/SUP], Zhihong Wu[SUP] 3 [/SUP], Hengrui Hu[SUP] 2 [/SUP], Yufeng Li[SUP] 2 [/SUP], Longda Ma[SUP] 7 [/SUP], Qian Liu[SUP] 7 [/SUP], Zhihong Hu[SUP] 2 [/SUP], Shuyang Zhang[SUP] 8 [/SUP], Yiwu Zhou[SUP] 7 [/SUP], Manli Wang[SUP] 2 [/SUP], Ling Leng[SUP] 3 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) patients with impact on skin and hair loss are reported. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is detected in the skin of some patients; however, the detailed pathological features of skin tissues from patients infected with SARS-CoV-2 at a molecular level are limited. Especially, the ability of SARS-CoV-2 to infect skin cells and impact their function is not well understood. A proteome map of COVID-19 skin is established here and the susceptibility of human-induced pluripotent stem cell (hiPSC)-derived skin organoids with hair follicles and nervous system is investigated, to SARS-CoV-2 infection. It is shown that KRT17+ hair follicles can be infected by SARS-CoV-2 and are associated with the impaired development of hair follicles and epidermis. Different types of nervous system cells are also found to be infected, which can lead to neuron death. Findings from the present work provide evidence for the association between COVID-19 and hair loss. hiPSC-derived skin organoids are also presented as an experimental model which can be used to investigate the susceptibility of skin cells to SARS-CoV-2 infection and can help identify various pathological mechanisms and drug screening strategies.
Keywords: COVID-19; SARS-CoV-2; hair follicle; nervous system; proteomics; skin organoids.
. 2021 Dec 31;e2104192.
doi: 10.1002/advs.202104192. Online ahead of print.
Establishment of Human Pluripotent Stem Cell-Derived Skin Organoids Enabled Pathophysiological Model of SARS-CoV-2 Infection
Jie Ma[SUP] 1 [/SUP], Jia Liu[SUP] 2 [/SUP], Dunqin Gao[SUP] 3 [/SUP], Xiao Li[SUP] 1 [/SUP], Qiyu Zhang[SUP] 4 [/SUP], Luye Lv[SUP] 5 [/SUP], Yujie Wang[SUP] 3 [/SUP], Jun Li[SUP] 4 [/SUP], Yunping Zhu[SUP] 1 6 [/SUP], Zhihong Wu[SUP] 3 [/SUP], Hengrui Hu[SUP] 2 [/SUP], Yufeng Li[SUP] 2 [/SUP], Longda Ma[SUP] 7 [/SUP], Qian Liu[SUP] 7 [/SUP], Zhihong Hu[SUP] 2 [/SUP], Shuyang Zhang[SUP] 8 [/SUP], Yiwu Zhou[SUP] 7 [/SUP], Manli Wang[SUP] 2 [/SUP], Ling Leng[SUP] 3 [/SUP]
Affiliations
- PMID: 34971128
- DOI: 10.1002/advs.202104192
Abstract
Coronavirus disease 2019 (COVID-19) patients with impact on skin and hair loss are reported. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is detected in the skin of some patients; however, the detailed pathological features of skin tissues from patients infected with SARS-CoV-2 at a molecular level are limited. Especially, the ability of SARS-CoV-2 to infect skin cells and impact their function is not well understood. A proteome map of COVID-19 skin is established here and the susceptibility of human-induced pluripotent stem cell (hiPSC)-derived skin organoids with hair follicles and nervous system is investigated, to SARS-CoV-2 infection. It is shown that KRT17+ hair follicles can be infected by SARS-CoV-2 and are associated with the impaired development of hair follicles and epidermis. Different types of nervous system cells are also found to be infected, which can lead to neuron death. Findings from the present work provide evidence for the association between COVID-19 and hair loss. hiPSC-derived skin organoids are also presented as an experimental model which can be used to investigate the susceptibility of skin cells to SARS-CoV-2 infection and can help identify various pathological mechanisms and drug screening strategies.
Keywords: COVID-19; SARS-CoV-2; hair follicle; nervous system; proteomics; skin organoids.