tetano
Editor, Senior Moderator
Aging (Albany NY). 2016 Nov 9. doi: 10.18632/aging.101105. [Epub ahead of print]
[h=1]Age-related changes in the gene expression profile of antigen-specific mouse CD8+ T cells can be partially reversed by blockade of the BTLA/CD160 pathways during vaccination.[/h] Dawany N[SUP]1,[/SUP][SUP]2[/SUP], Parzych EM[SUP]1,[/SUP][SUP]3[/SUP], Showe LC[SUP]1[/SUP], Ertl HC[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We analyzed gene expression profiles of young and aged mouse CD8[SUP]+[/SUP] T cells specific for the nucleoprotein (NP) of influenza A/PR8/34 virus. CD8[SUP]+[/SUP] T cells were stimulated either by the NP antigen expressed in its native form or fused into the herpes virus (HSV)-1 glycoprotein D (gD) protein, which blocks signaling through the immunoinhibitory B and T lymphocyte attenuator (BTLA) and CD160 pathways. We show that NP-specific CD8[SUP]+[/SUP] T cells from aged mice exhibit numerous differences in gene expression compared to NP-specific CD8[SUP]+[/SUP] T cells from young mice, including a significant reduction of expression in genes involved in T cell receptor (TcR) and CD28 signaling. We also show that these changes can be reversed in a sub-population (~50%) of the aged mice by a BTLA/CD160 checkpoint blockade. These results suggest that BTLA/CD160 checkpoint blockade has potential value as a vaccine additive to induce better CD8[SUP]+[/SUP] T cell responses in the aged.
[h=4]KEYWORDS:[/h] BTLA/CD160; CD8+ cells; aging; gene expression; vaccination
PMID: 27922818 DOI: 10.18632/aging.101105
[PubMed - as supplied by publisher]
[h=1]Age-related changes in the gene expression profile of antigen-specific mouse CD8+ T cells can be partially reversed by blockade of the BTLA/CD160 pathways during vaccination.[/h] Dawany N[SUP]1,[/SUP][SUP]2[/SUP], Parzych EM[SUP]1,[/SUP][SUP]3[/SUP], Showe LC[SUP]1[/SUP], Ertl HC[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We analyzed gene expression profiles of young and aged mouse CD8[SUP]+[/SUP] T cells specific for the nucleoprotein (NP) of influenza A/PR8/34 virus. CD8[SUP]+[/SUP] T cells were stimulated either by the NP antigen expressed in its native form or fused into the herpes virus (HSV)-1 glycoprotein D (gD) protein, which blocks signaling through the immunoinhibitory B and T lymphocyte attenuator (BTLA) and CD160 pathways. We show that NP-specific CD8[SUP]+[/SUP] T cells from aged mice exhibit numerous differences in gene expression compared to NP-specific CD8[SUP]+[/SUP] T cells from young mice, including a significant reduction of expression in genes involved in T cell receptor (TcR) and CD28 signaling. We also show that these changes can be reversed in a sub-population (~50%) of the aged mice by a BTLA/CD160 checkpoint blockade. These results suggest that BTLA/CD160 checkpoint blockade has potential value as a vaccine additive to induce better CD8[SUP]+[/SUP] T cell responses in the aged.
[h=4]KEYWORDS:[/h] BTLA/CD160; CD8+ cells; aging; gene expression; vaccination
PMID: 27922818 DOI: 10.18632/aging.101105
[PubMed - as supplied by publisher]