tetano
Editor, Senior Moderator
Am J Clin Pathol
. 2020 Aug 18;aqaa140.
doi: 10.1093/ajcp/aqaa140. Online ahead of print.
Humoral Immune Response to SARS-CoV-2
Pauline H Herroelen[SUP] 1 [/SUP], Geert A Martens[SUP] 1 2 3 [/SUP], Dieter De Smet[SUP] 1 [/SUP], Koen Swaerts[SUP] 1 [/SUP], An-Sofie Decavele[SUP] 1 [/SUP]
Affiliations
Abstract
Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests are clinically useful to document prior SARS-CoV-2 infections. Data are urgently needed to select assays with optimal sensitivity at acceptable specificity for antibody detection.
Methods: A comparative evaluation was performed of 7 commercial SARS-CoV-2 serology assays on 171 sera from 135 subjects with polymerase chain reaction-confirmed SARS-CoV-2 infection (71 hospitalized patients and 64 paucisymptomatic individuals). Kinetics of IgA/IgM/IgG seroconversion to viral N and S protein epitopes were studied from 0 to 54 days after onset of symptoms. Cross-reactivity was verified on 57 prepandemic samples.
Results: Wantai SARS-COV-2 Ab ELISA and Orient Gene COVID-19 IgG/IgM Rapid Test showed superior overall sensitivity for detection of SARS-CoV-2 antibodies. Elecsys Anti-SARS-CoV-2 assay and EUROIMMUN Anti-SARS-CoV-2 combined IgG/IgA showed acceptable sensitivity (>95%) vs the consensus result of all assays from 10 days post onset of symptoms. Wantai SARS-COV-2 Ab ELISA, Elecsys Anti-SARS-CoV-2 assay, and Innovita 2019-nCoV Ab rapid test showed least cross-reactivity, resulting in an optimal analytical specificity greater than 98%.
Conclusions: Wantai SARS-COV-2 Ab ELISA and Elecsys Anti-SARS-CoV-2 assays are suitable for sensitive and specific detection of SARS-CoV-2 antibodies from 10 days after onset of symptoms.
Keywords: Antibody; COVID-19; Coronavirus; Paucisymptomatic; SARS-CoV-2; Serology.
. 2020 Aug 18;aqaa140.
doi: 10.1093/ajcp/aqaa140. Online ahead of print.
Humoral Immune Response to SARS-CoV-2
Pauline H Herroelen[SUP] 1 [/SUP], Geert A Martens[SUP] 1 2 3 [/SUP], Dieter De Smet[SUP] 1 [/SUP], Koen Swaerts[SUP] 1 [/SUP], An-Sofie Decavele[SUP] 1 [/SUP]
Affiliations
- PMID: 32808976
- DOI: 10.1093/ajcp/aqaa140
Abstract
Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests are clinically useful to document prior SARS-CoV-2 infections. Data are urgently needed to select assays with optimal sensitivity at acceptable specificity for antibody detection.
Methods: A comparative evaluation was performed of 7 commercial SARS-CoV-2 serology assays on 171 sera from 135 subjects with polymerase chain reaction-confirmed SARS-CoV-2 infection (71 hospitalized patients and 64 paucisymptomatic individuals). Kinetics of IgA/IgM/IgG seroconversion to viral N and S protein epitopes were studied from 0 to 54 days after onset of symptoms. Cross-reactivity was verified on 57 prepandemic samples.
Results: Wantai SARS-COV-2 Ab ELISA and Orient Gene COVID-19 IgG/IgM Rapid Test showed superior overall sensitivity for detection of SARS-CoV-2 antibodies. Elecsys Anti-SARS-CoV-2 assay and EUROIMMUN Anti-SARS-CoV-2 combined IgG/IgA showed acceptable sensitivity (>95%) vs the consensus result of all assays from 10 days post onset of symptoms. Wantai SARS-COV-2 Ab ELISA, Elecsys Anti-SARS-CoV-2 assay, and Innovita 2019-nCoV Ab rapid test showed least cross-reactivity, resulting in an optimal analytical specificity greater than 98%.
Conclusions: Wantai SARS-COV-2 Ab ELISA and Elecsys Anti-SARS-CoV-2 assays are suitable for sensitive and specific detection of SARS-CoV-2 antibodies from 10 days after onset of symptoms.
Keywords: Antibody; COVID-19; Coronavirus; Paucisymptomatic; SARS-CoV-2; Serology.