tetano
Editor, Senior Moderator
Am J Physiol Lung Cell Mol Physiol
. 2020 Nov 4.
doi: 10.1152/ajplung.00355.2020. Online ahead of print.
High-dimensional single-cell analysis reveals the immune characteristics of COVID-19
Wen Shi[SUP] 1 [/SUP], Xiuxing Liu[SUP] 1 [/SUP], Qiqi Cao[SUP] 2 [/SUP], Pengjuan Ma[SUP] 1 [/SUP], Wenqing Le[SUP] 3 [/SUP], Lihui Xie[SUP] 1 [/SUP], JinGuo Ye[SUP] 1 [/SUP], Wen Wen[SUP] 4 [/SUP], Hao Tang[SUP] 5 [/SUP], Wenru Su[SUP] 1 [/SUP], Yingfeng Zheng[SUP] 1 [/SUP], Yizhi Liu[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19), driven by SARS-CoV-2, is now declared a global pandemic. Pathogenic T cells and inflammatory monocytes are regarded as the central drivers of the cytokine storm associated with the severity of COVID-19. In this study, we explored the characteristic peripheral cellular profiles of patients with COVID-19 in both acute and convalescent phases by single-cell mass cytometry (CyTOF). Using a combination of algorithm-guided data analyses, we identified peripheral immune cell subsets in COVID-19, and revealed CD4[SUP]+[/SUP] T cell depletion, T cell differentiation, plasma cell expansion, and the reduced antigen presentation capacity of innate immunity. Notably, COVID-19 induces a dysregulation in the balance of monocyte populations by the expansion of the monocyte subsets. Collectively, our results represent a high-dimensional single-cell profile of the peripheral immune response to SARS-CoV-2 infection.
Keywords: Acute phase; COVID-19; CyTOF; Peripheral blood mononuclear cell; SARS-CoV-2.
. 2020 Nov 4.
doi: 10.1152/ajplung.00355.2020. Online ahead of print.
High-dimensional single-cell analysis reveals the immune characteristics of COVID-19
Wen Shi[SUP] 1 [/SUP], Xiuxing Liu[SUP] 1 [/SUP], Qiqi Cao[SUP] 2 [/SUP], Pengjuan Ma[SUP] 1 [/SUP], Wenqing Le[SUP] 3 [/SUP], Lihui Xie[SUP] 1 [/SUP], JinGuo Ye[SUP] 1 [/SUP], Wen Wen[SUP] 4 [/SUP], Hao Tang[SUP] 5 [/SUP], Wenru Su[SUP] 1 [/SUP], Yingfeng Zheng[SUP] 1 [/SUP], Yizhi Liu[SUP] 1 [/SUP]
Affiliations
- PMID: 33146564
- DOI: 10.1152/ajplung.00355.2020
Abstract
Coronavirus disease 2019 (COVID-19), driven by SARS-CoV-2, is now declared a global pandemic. Pathogenic T cells and inflammatory monocytes are regarded as the central drivers of the cytokine storm associated with the severity of COVID-19. In this study, we explored the characteristic peripheral cellular profiles of patients with COVID-19 in both acute and convalescent phases by single-cell mass cytometry (CyTOF). Using a combination of algorithm-guided data analyses, we identified peripheral immune cell subsets in COVID-19, and revealed CD4[SUP]+[/SUP] T cell depletion, T cell differentiation, plasma cell expansion, and the reduced antigen presentation capacity of innate immunity. Notably, COVID-19 induces a dysregulation in the balance of monocyte populations by the expansion of the monocyte subsets. Collectively, our results represent a high-dimensional single-cell profile of the peripheral immune response to SARS-CoV-2 infection.
Keywords: Acute phase; COVID-19; CyTOF; Peripheral blood mononuclear cell; SARS-CoV-2.