tetano
Editor, Senior Moderator
Arch Virol. 2016 Mar 21. [Epub ahead of print]
[h=1]Amino acid substitutions occurring during adaptation of an emergent H5N6 avian influenza virus to mammals.[/h] Peng X[SUP]1[/SUP], Wu H[SUP]1[/SUP], Peng X[SUP]1[/SUP], Wu X[SUP]1[/SUP], Cheng L[SUP]1[/SUP], Liu F[SUP]1[/SUP], Ji S[SUP]1[/SUP], Wu N[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza viruses (AIVs) are known to cross species barriers, and emergent highly pathogenic H5N6 AIVs pose a serious threat to human health and the poultry industry. Here, we serially passaged an H5N6 virus 10 times in BALB/c mice. The pathogenicity of the wild-type 6D2 (WT-6D2) and mammal-adapted 6D2 strain (MA-6D2) were compared. The viral titer in multiple organs and the death rate for MA-6D2 were significantly higher than for WT-6D2. We provide evidence that the mutations HA A150V, NA R143K and G147E, PB2 E627K, and PA A343T may be important for adaptation of H5N6 AIVs to mammals.
[h=4]KEYWORDS:[/h] Avian influenza virus; H5N6; Mammal adaption; Pathogenicity; Serial passage
PMID: 26997612 [PubMed - as supplied by publisher]
[h=1]Amino acid substitutions occurring during adaptation of an emergent H5N6 avian influenza virus to mammals.[/h] Peng X[SUP]1[/SUP], Wu H[SUP]1[/SUP], Peng X[SUP]1[/SUP], Wu X[SUP]1[/SUP], Cheng L[SUP]1[/SUP], Liu F[SUP]1[/SUP], Ji S[SUP]1[/SUP], Wu N[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza viruses (AIVs) are known to cross species barriers, and emergent highly pathogenic H5N6 AIVs pose a serious threat to human health and the poultry industry. Here, we serially passaged an H5N6 virus 10 times in BALB/c mice. The pathogenicity of the wild-type 6D2 (WT-6D2) and mammal-adapted 6D2 strain (MA-6D2) were compared. The viral titer in multiple organs and the death rate for MA-6D2 were significantly higher than for WT-6D2. We provide evidence that the mutations HA A150V, NA R143K and G147E, PB2 E627K, and PA A343T may be important for adaptation of H5N6 AIVs to mammals.
[h=4]KEYWORDS:[/h] Avian influenza virus; H5N6; Mammal adaption; Pathogenicity; Serial passage
PMID: 26997612 [PubMed - as supplied by publisher]