tetano
Editor, Senior Moderator
J Immunol Methods. 2014 Nov 7. pii: S0022-1759(14)00298-1. doi: 10.1016/j.jim.2014.10.012. [Epub ahead of print]
An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination.
Ruiz-Hernandez R1, Peroval M1, Boyd A2, Balkissoon D1, Staines K1, Smith A2, Butter C3.
Author information
Abstract
A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFNγ producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein.
Copyright ? 2014 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:
Adaptive antiviral responses; Antigen presentation; Avian influenza; CD8 T cell; IFNγ ELISpot
PMID:
25450002
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25450002
An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination.
Ruiz-Hernandez R1, Peroval M1, Boyd A2, Balkissoon D1, Staines K1, Smith A2, Butter C3.
Author information
Abstract
A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFNγ producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein.
Copyright ? 2014 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:
Adaptive antiviral responses; Antigen presentation; Avian influenza; CD8 T cell; IFNγ ELISpot
PMID:
25450002
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25450002