tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2015 Jun 24. pii: CVI.00278-15. [Epub ahead of print]
[h=1]An international laboratory comparison of influenza microneutralisation assay protocols for A(H1N1)pdm09, A(H3N2) and A(H5N1) influenza A viruses by CONSISE.[/h] Laurie KL[SUP]1[/SUP], Engelhardt OG[SUP]2[/SUP], Wood J[SUP]3[/SUP], Heath A[SUP]3[/SUP], Katz JM[SUP]4[/SUP], Peiris M[SUP]5[/SUP], Hoschler K[SUP]6[/SUP], Hungnes O[SUP]7[/SUP], Zhang W[SUP]8[/SUP], Van Kerkhove MD[SUP]9[/SUP]; CONSISE Laboratory Working Group participants.
[h=3]Author information[/h]
[h=3]Abstract[/h] The Microneutralisation assay is commonly used to detect antibodies to influenza virus and multiple protocols are used worldwide. These protocols vary in the incubation time of the assay as well as in the order of specific steps and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus a laboratory comparison of the 2-day ELISA and 3-day hemagglutination (HA) microneutralisation (MN) protocols, using A(H1N1)pdm09, A(H3N2) and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titres for each assay protocol were compared to determine the sensitivity of each assay and between replicates to assess the reproducibility of each protocol, for each laboratory. There was good correlation between the results obtained using the two assay protocols in most laboratories, indicating these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodology to the CONSISE Consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26108286 [PubMed - as supplied by publisher]
[h=1]An international laboratory comparison of influenza microneutralisation assay protocols for A(H1N1)pdm09, A(H3N2) and A(H5N1) influenza A viruses by CONSISE.[/h] Laurie KL[SUP]1[/SUP], Engelhardt OG[SUP]2[/SUP], Wood J[SUP]3[/SUP], Heath A[SUP]3[/SUP], Katz JM[SUP]4[/SUP], Peiris M[SUP]5[/SUP], Hoschler K[SUP]6[/SUP], Hungnes O[SUP]7[/SUP], Zhang W[SUP]8[/SUP], Van Kerkhove MD[SUP]9[/SUP]; CONSISE Laboratory Working Group participants.
[h=3]Author information[/h]
[h=3]Abstract[/h] The Microneutralisation assay is commonly used to detect antibodies to influenza virus and multiple protocols are used worldwide. These protocols vary in the incubation time of the assay as well as in the order of specific steps and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus a laboratory comparison of the 2-day ELISA and 3-day hemagglutination (HA) microneutralisation (MN) protocols, using A(H1N1)pdm09, A(H3N2) and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titres for each assay protocol were compared to determine the sensitivity of each assay and between replicates to assess the reproducibility of each protocol, for each laboratory. There was good correlation between the results obtained using the two assay protocols in most laboratories, indicating these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodology to the CONSISE Consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26108286 [PubMed - as supplied by publisher]