tetano
Editor, Senior Moderator
Anal Biochem
. 2024 Feb 29:689:115500.
doi: 10.1016/j.ab.2024.115500. Online ahead of print. COVID-19 electrochemical immunosensor with Ag-MOF: Rapid and high-selectivity nasal swab testing for effective detection
Sadra Adel[SUP] 1 [/SUP], Ali Firoozbakhtian[SUP] 2 [/SUP], Hodjattallah Rabbani[SUP] 3 [/SUP], Morteza Hosseini[SUP] 4 [/SUP], Azam Bagheri Pebdeni[SUP] 2 [/SUP], Niloufar Sadeghi[SUP] 3 [/SUP], Javad Gilnezhad[SUP] 1 [/SUP], Mohammad Reza Ganjali[SUP] 5 [/SUP]
Affiliations
Early detection of the coronavirus is acknowledged as a crucial measure to mitigate the spread of the pandemic, facilitating timely isolation of infected individuals, and disrupting the transmission chain. In this study, we leveraged the properties of synthesized Ag-MOF, including high porosity and increased flow intensity. Electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed to develop an economical and portable sensor with exceptional selectivity for COVID-19 detection. The methodology involves the deposition of Ag-MOF onto the surface of a Glassy Carbon Electrode (GCE), which resulted in a progressive augmentation of electric current. Subsequently, the targeted antibodies were applied, and relevant tests were conducted. The sensor demonstrated the capacity to detect the virus within a linear range of 100 fM to 10 nM, boasting a noteworthy Limit of Detection (LOD) of 60 fM. The entire detection process could be completed in a brief duration of 20 min, exhibiting high levels of accuracy and precision, outperforming comparable techniques in terms of speed and efficacy.
Keywords: Antibody; COVID-19; Electrochemistry; Immunosensing; MOF.
. 2024 Feb 29:689:115500.
doi: 10.1016/j.ab.2024.115500. Online ahead of print. COVID-19 electrochemical immunosensor with Ag-MOF: Rapid and high-selectivity nasal swab testing for effective detection
Sadra Adel[SUP] 1 [/SUP], Ali Firoozbakhtian[SUP] 2 [/SUP], Hodjattallah Rabbani[SUP] 3 [/SUP], Morteza Hosseini[SUP] 4 [/SUP], Azam Bagheri Pebdeni[SUP] 2 [/SUP], Niloufar Sadeghi[SUP] 3 [/SUP], Javad Gilnezhad[SUP] 1 [/SUP], Mohammad Reza Ganjali[SUP] 5 [/SUP]
Affiliations
- PMID: 38431139
- DOI: 10.1016/j.ab.2024.115500
Early detection of the coronavirus is acknowledged as a crucial measure to mitigate the spread of the pandemic, facilitating timely isolation of infected individuals, and disrupting the transmission chain. In this study, we leveraged the properties of synthesized Ag-MOF, including high porosity and increased flow intensity. Electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed to develop an economical and portable sensor with exceptional selectivity for COVID-19 detection. The methodology involves the deposition of Ag-MOF onto the surface of a Glassy Carbon Electrode (GCE), which resulted in a progressive augmentation of electric current. Subsequently, the targeted antibodies were applied, and relevant tests were conducted. The sensor demonstrated the capacity to detect the virus within a linear range of 100 fM to 10 nM, boasting a noteworthy Limit of Detection (LOD) of 60 fM. The entire detection process could be completed in a brief duration of 20 min, exhibiting high levels of accuracy and precision, outperforming comparable techniques in terms of speed and efficacy.
Keywords: Antibody; COVID-19; Electrochemistry; Immunosensing; MOF.