tetano
Editor, Senior Moderator
Anal Chem
. 2023 Mar 22.
doi: 10.1021/acs.analchem.2c04273. Online ahead of print.
Discrimination of the H1N1 and H5N2 Variants of Influenza A Virus Using an Isomeric Sialic Acid-Conjugated Graphene Field-Effect Transistor
Sophia Nazir[SUP] 1 2 [/SUP], Kyung Ho Kim[SUP] 1 [/SUP], Lina Kim[SUP] 1 [/SUP], Sung Eun Seo[SUP] 1 [/SUP], Pan Kee Bae[SUP] 3 [/SUP], Jai Eun An[SUP] 1 [/SUP], Oh Seok Kwon[SUP] 4 5 1 2 [/SUP]
Affiliations
Abstract
There has been a continuous effort to fabricate a fast, sensitive, and inexpensive system for influenza virus detection to meet the demand for effective screening in point-of-care testing. Herein, we report a sialic acid (SA)-conjugated graphene field-effect transistor (SA-GFET) sensor designed using α2,3-linked sialic acid (3'-SA) and α2,6-linked sialic acid (6'-SA) for the detection and discrimination of the hemagglutinin (HA) protein of the H5N2 and H1N1 viruses. 3'-SA and 6'-SA specific for H5 and H1 influenza were used in the SA-GFET to capture the HA protein of the influenza virus. The net charge of the captured viral sample led to a change in the electrical current of the SA-GFET platform, which could be correlated to the concentration of the viral sample. This SA-GFET platform exhibited a highly sensitive response in the range of 10[SUP]1[/SUP]-10[SUP]6[/SUP] pfu mL[SUP]-1[/SUP], with a limit of detection (LOD) of 10[SUP]1[/SUP] pfu mL[SUP]-1[/SUP] in buffer solution and a response time of approximately 10 s. The selectivity of the SA-GFET platform for the H1N1 and H5N2 influenza viruses was verified by testing analogous respiratory viruses, i.e., influenza B and the spike protein of SARS-CoV-2 and MERS-CoV, on the SA-GFET. Overall, the results demonstrate that the developed dual-channel SA-GFET platform can potentially serve as a highly efficient and sensitive sensing platform for the rapid detection of infectious diseases.
. 2023 Mar 22.
doi: 10.1021/acs.analchem.2c04273. Online ahead of print.
Discrimination of the H1N1 and H5N2 Variants of Influenza A Virus Using an Isomeric Sialic Acid-Conjugated Graphene Field-Effect Transistor
Sophia Nazir[SUP] 1 2 [/SUP], Kyung Ho Kim[SUP] 1 [/SUP], Lina Kim[SUP] 1 [/SUP], Sung Eun Seo[SUP] 1 [/SUP], Pan Kee Bae[SUP] 3 [/SUP], Jai Eun An[SUP] 1 [/SUP], Oh Seok Kwon[SUP] 4 5 1 2 [/SUP]
Affiliations
- PMID: 36947869
- DOI: 10.1021/acs.analchem.2c04273
Abstract
There has been a continuous effort to fabricate a fast, sensitive, and inexpensive system for influenza virus detection to meet the demand for effective screening in point-of-care testing. Herein, we report a sialic acid (SA)-conjugated graphene field-effect transistor (SA-GFET) sensor designed using α2,3-linked sialic acid (3'-SA) and α2,6-linked sialic acid (6'-SA) for the detection and discrimination of the hemagglutinin (HA) protein of the H5N2 and H1N1 viruses. 3'-SA and 6'-SA specific for H5 and H1 influenza were used in the SA-GFET to capture the HA protein of the influenza virus. The net charge of the captured viral sample led to a change in the electrical current of the SA-GFET platform, which could be correlated to the concentration of the viral sample. This SA-GFET platform exhibited a highly sensitive response in the range of 10[SUP]1[/SUP]-10[SUP]6[/SUP] pfu mL[SUP]-1[/SUP], with a limit of detection (LOD) of 10[SUP]1[/SUP] pfu mL[SUP]-1[/SUP] in buffer solution and a response time of approximately 10 s. The selectivity of the SA-GFET platform for the H1N1 and H5N2 influenza viruses was verified by testing analogous respiratory viruses, i.e., influenza B and the spike protein of SARS-CoV-2 and MERS-CoV, on the SA-GFET. Overall, the results demonstrate that the developed dual-channel SA-GFET platform can potentially serve as a highly efficient and sensitive sensing platform for the rapid detection of infectious diseases.