tetano
Editor, Senior Moderator
Anal Chem
. 2022 Mar 3.
doi: 10.1021/acs.analchem.1c04328. Online ahead of print.
Matrix-Assisted Laser Desorption and Ionization Time-of-Flight Mass Spectrometry Analysis for the Direct Detection of SARS-CoV-2 in Nasopharyngeal Swabs
Tomoya Yoshinari[SUP] 1 [/SUP], Katsuhiko Hayashi[SUP] 1 [/SUP], Shouhei Hirose[SUP] 1 [/SUP], Kenji Ohya[SUP] 1 [/SUP], Takahiro Ohnishi[SUP] 1 [/SUP], Maiko Watanabe[SUP] 1 [/SUP], Satoshi Taharaguchi[SUP] 2 [/SUP], Hirohisa Mekata[SUP] 3 [/SUP], Takahide Taniguchi[SUP] 4 [/SUP], Takuya Maeda[SUP] 5 [/SUP], Yuta Orihara[SUP] 5 [/SUP], Rieko Kawamura[SUP] 5 [/SUP], Sakura Arai[SUP] 1 [/SUP], Yoshiro Saito[SUP] 6 [/SUP], Yukihiro Goda[SUP] 7 [/SUP], Yukiko Hara-Kudo[SUP] 1 [/SUP]
Affiliations
Abstract
The most common diagnostic method used for coronavirus disease-2019 (COVID-19) is real-time reverse transcription polymerase chain reaction (PCR). However, it requires complex and labor-intensive procedures and involves excessive positive results derived from viral debris. We developed a method for the direct detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swabs, which uses matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-ToF MS) to identify specific peptides from the SARS-CoV-2 nucleocapsid phosphoprotein (NP). SARS-CoV-2 viral particles were separated from biological molecules in nasopharyngeal swabs by an ultrafiltration cartridge. Further purification was performed by an anion exchange resin, and purified NP was digested into peptides using trypsin. The peptides from SARS-CoV-2 that were inoculated into nasopharyngeal swabs were detected by MALDI-ToF MS, and the limit of detection was 10[SUP]6.7[/SUP] viral copies. This value equates to 10[SUP]7.9[/SUP] viral copies per swab and is approximately equivalent to the viral load of contagious patients. Seven NP-derived peptides were selected as the target molecules for the detection of SARS-CoV-2 in clinical specimens. The method detected between two and seven NP-derived peptides in 19 nasopharyngeal swab specimens from contagious COVID-19 patients. These peptides were not detected in four specimens in which SARS-CoV-2 RNA was not detected by PCR. Mutated NP-derived peptides were found in some specimens, and their patterns of amino acid replacement were estimated by accurate mass. Our results provide evidence that the developed MALDI-ToF MS-based method in a combination of straightforward purification steps and a rapid detection step directly detect SARS-CoV-2-specific peptides in nasopharyngeal swabs and can be a reliable high-throughput diagnostic method for COVID-19.
. 2022 Mar 3.
doi: 10.1021/acs.analchem.1c04328. Online ahead of print.
Matrix-Assisted Laser Desorption and Ionization Time-of-Flight Mass Spectrometry Analysis for the Direct Detection of SARS-CoV-2 in Nasopharyngeal Swabs
Tomoya Yoshinari[SUP] 1 [/SUP], Katsuhiko Hayashi[SUP] 1 [/SUP], Shouhei Hirose[SUP] 1 [/SUP], Kenji Ohya[SUP] 1 [/SUP], Takahiro Ohnishi[SUP] 1 [/SUP], Maiko Watanabe[SUP] 1 [/SUP], Satoshi Taharaguchi[SUP] 2 [/SUP], Hirohisa Mekata[SUP] 3 [/SUP], Takahide Taniguchi[SUP] 4 [/SUP], Takuya Maeda[SUP] 5 [/SUP], Yuta Orihara[SUP] 5 [/SUP], Rieko Kawamura[SUP] 5 [/SUP], Sakura Arai[SUP] 1 [/SUP], Yoshiro Saito[SUP] 6 [/SUP], Yukihiro Goda[SUP] 7 [/SUP], Yukiko Hara-Kudo[SUP] 1 [/SUP]
Affiliations
- PMID: 35238540
- DOI: 10.1021/acs.analchem.1c04328
Abstract
The most common diagnostic method used for coronavirus disease-2019 (COVID-19) is real-time reverse transcription polymerase chain reaction (PCR). However, it requires complex and labor-intensive procedures and involves excessive positive results derived from viral debris. We developed a method for the direct detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swabs, which uses matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-ToF MS) to identify specific peptides from the SARS-CoV-2 nucleocapsid phosphoprotein (NP). SARS-CoV-2 viral particles were separated from biological molecules in nasopharyngeal swabs by an ultrafiltration cartridge. Further purification was performed by an anion exchange resin, and purified NP was digested into peptides using trypsin. The peptides from SARS-CoV-2 that were inoculated into nasopharyngeal swabs were detected by MALDI-ToF MS, and the limit of detection was 10[SUP]6.7[/SUP] viral copies. This value equates to 10[SUP]7.9[/SUP] viral copies per swab and is approximately equivalent to the viral load of contagious patients. Seven NP-derived peptides were selected as the target molecules for the detection of SARS-CoV-2 in clinical specimens. The method detected between two and seven NP-derived peptides in 19 nasopharyngeal swab specimens from contagious COVID-19 patients. These peptides were not detected in four specimens in which SARS-CoV-2 RNA was not detected by PCR. Mutated NP-derived peptides were found in some specimens, and their patterns of amino acid replacement were estimated by accurate mass. Our results provide evidence that the developed MALDI-ToF MS-based method in a combination of straightforward purification steps and a rapid detection step directly detect SARS-CoV-2-specific peptides in nasopharyngeal swabs and can be a reliable high-throughput diagnostic method for COVID-19.