tetano
Editor, Senior Moderator
Anal Chim Acta
. 2023 Jan 15;1238:340633.
doi: 10.1016/j.aca.2022.340633. Epub 2022 Nov 16.
A luciferase based automated assay for rapid and sensitive detection of SARS-CoV-2 antibodies
Wenhao Zhou[SUP] 1 [/SUP], Ping He[SUP] 1 [/SUP], Huan Liu[SUP] 1 [/SUP], Hongping Wei[SUP] 2 [/SUP], Junping Yu[SUP] 3 [/SUP]
Affiliations
Abstract
The Coronavirus disease 2019 (COVID-19) pandemic brings great challenges to the public health and social economics around the world. As the pandemic continues and the mass vaccination goes on, monitoring the antibodies is particularly important for the epidemiological survey and vaccine assessment. Here, we developed a luciferase immunoprecipitation assay combined with an automated platform to detect anti-Receptor Binding Domain (RBD) antibody, where protein A and protein G modified magnetic beads were used to capture antibodies in serum samples and SARS-CoV-2 RBD was fused with Gaussia luciferase to label the captured target antibodies. The whole detection procedure can be completed within 20 min. The developed assay has proven up to 32 times more sensitive than ELISA for the detection of RBD antibodies. Furthermore, the results of the antibody detection of sera from vaccination as well as convalescence displayed good performance. The automated platform may provide a powerful tool for the control of COVID-19 pandemic by vaccination and the research of SARS-CoV-2 seroconversion.
Keywords: Antibody detection; Gaussia luciferase; Luciferase immunoprecipitation system; Receptor binding domain; SARS-CoV-2.
. 2023 Jan 15;1238:340633.
doi: 10.1016/j.aca.2022.340633. Epub 2022 Nov 16.
A luciferase based automated assay for rapid and sensitive detection of SARS-CoV-2 antibodies
Wenhao Zhou[SUP] 1 [/SUP], Ping He[SUP] 1 [/SUP], Huan Liu[SUP] 1 [/SUP], Hongping Wei[SUP] 2 [/SUP], Junping Yu[SUP] 3 [/SUP]
Affiliations
- PMID: 36464447
- DOI: 10.1016/j.aca.2022.340633
Abstract
The Coronavirus disease 2019 (COVID-19) pandemic brings great challenges to the public health and social economics around the world. As the pandemic continues and the mass vaccination goes on, monitoring the antibodies is particularly important for the epidemiological survey and vaccine assessment. Here, we developed a luciferase immunoprecipitation assay combined with an automated platform to detect anti-Receptor Binding Domain (RBD) antibody, where protein A and protein G modified magnetic beads were used to capture antibodies in serum samples and SARS-CoV-2 RBD was fused with Gaussia luciferase to label the captured target antibodies. The whole detection procedure can be completed within 20 min. The developed assay has proven up to 32 times more sensitive than ELISA for the detection of RBD antibodies. Furthermore, the results of the antibody detection of sera from vaccination as well as convalescence displayed good performance. The automated platform may provide a powerful tool for the control of COVID-19 pandemic by vaccination and the research of SARS-CoV-2 seroconversion.
Keywords: Antibody detection; Gaussia luciferase; Luciferase immunoprecipitation system; Receptor binding domain; SARS-CoV-2.