tetano
Editor, Senior Moderator
Vet Microbiol. 2016 Jan 15;182:1-7. doi: 10.1016/j.vetmic.2015.10.031. Epub 2015 Nov 2.
[h=1]Antigenic evolution of H9N2 chicken influenza viruses isolated in China during 2009-2013 and selection of a candidate vaccine strain with broad cross-reactivity.[/h] Wei Y[SUP]1[/SUP], Xu G[SUP]1[/SUP], Zhang G[SUP]1[/SUP], Wen C[SUP]1[/SUP], Anwar F[SUP]2[/SUP], Wang S[SUP]3[/SUP], Lemmon G[SUP]4[/SUP], Wang J[SUP]1[/SUP], Carter R[SUP]3[/SUP], Wang M[SUP]1[/SUP], Sun H[SUP]1[/SUP], Sun Y[SUP]1[/SUP], Zhao J[SUP]1[/SUP], Wu G[SUP]3[/SUP], Webster RG[SUP]5[/SUP], Liu J[SUP]1[/SUP], Pu J[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We previously demonstrated that H9N2 subtype avian influenza viruses (AIVs) isolated from 1994 to 2008 evolved into distinct antigenic groups (C, D, and E) and then underwent antigenic drift from commercial vaccines, causing a country-wide outbreak during 2010-2013. In this study, H9N2 AIVs isolated from chickens during 2009-2013 were antigenically analyzed by performing hemagglutination inhibition and neutralization assays using a panel of polyclonal antibodies. Our findings confirmed the antigenic drift of recent H9N2 viruses from the commercial vaccine and showed that most of these antigenic variants form a novel HI antigenic group, F, with a few belonging to groups D and E. Slight antigenic variation was observed in group F viruses. Genetic analysis of amino acid sequences deduced from hemagglutinin (HA) gene sequences indicated that 9 of 15 mutations predominant in the 2009-2013 viruses can be mapped to known antigenic sites, which might be responsible for the novel antigenicity of group F. These antigenic changes make it necessary to modify the influenza vaccine to ensure efficient protection. A vaccine candidate, Ck/HeB/YT/10, was selected and provided significant protection against viruses from different antigenic groups in terms of reduction in virus shedding, suggesting broad cross-reactivity. Taken together, our results indicate that the H9N2 chicken influenza viruses in China have evolved from distinct antigenic groups into a novel group F that became dominant during the country-wide outbreak and now seems to be undergoing new antigenic divergence. Systematic surveillance and timely updating of vaccine strains are important for viral prevention and control in the future.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic evolution; Chicken influenza virus; H9N2; Vaccine
PMID: 26711021 [PubMed - in process]
[h=1]Antigenic evolution of H9N2 chicken influenza viruses isolated in China during 2009-2013 and selection of a candidate vaccine strain with broad cross-reactivity.[/h] Wei Y[SUP]1[/SUP], Xu G[SUP]1[/SUP], Zhang G[SUP]1[/SUP], Wen C[SUP]1[/SUP], Anwar F[SUP]2[/SUP], Wang S[SUP]3[/SUP], Lemmon G[SUP]4[/SUP], Wang J[SUP]1[/SUP], Carter R[SUP]3[/SUP], Wang M[SUP]1[/SUP], Sun H[SUP]1[/SUP], Sun Y[SUP]1[/SUP], Zhao J[SUP]1[/SUP], Wu G[SUP]3[/SUP], Webster RG[SUP]5[/SUP], Liu J[SUP]1[/SUP], Pu J[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We previously demonstrated that H9N2 subtype avian influenza viruses (AIVs) isolated from 1994 to 2008 evolved into distinct antigenic groups (C, D, and E) and then underwent antigenic drift from commercial vaccines, causing a country-wide outbreak during 2010-2013. In this study, H9N2 AIVs isolated from chickens during 2009-2013 were antigenically analyzed by performing hemagglutination inhibition and neutralization assays using a panel of polyclonal antibodies. Our findings confirmed the antigenic drift of recent H9N2 viruses from the commercial vaccine and showed that most of these antigenic variants form a novel HI antigenic group, F, with a few belonging to groups D and E. Slight antigenic variation was observed in group F viruses. Genetic analysis of amino acid sequences deduced from hemagglutinin (HA) gene sequences indicated that 9 of 15 mutations predominant in the 2009-2013 viruses can be mapped to known antigenic sites, which might be responsible for the novel antigenicity of group F. These antigenic changes make it necessary to modify the influenza vaccine to ensure efficient protection. A vaccine candidate, Ck/HeB/YT/10, was selected and provided significant protection against viruses from different antigenic groups in terms of reduction in virus shedding, suggesting broad cross-reactivity. Taken together, our results indicate that the H9N2 chicken influenza viruses in China have evolved from distinct antigenic groups into a novel group F that became dominant during the country-wide outbreak and now seems to be undergoing new antigenic divergence. Systematic surveillance and timely updating of vaccine strains are important for viral prevention and control in the future.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic evolution; Chicken influenza virus; H9N2; Vaccine
PMID: 26711021 [PubMed - in process]