tetano
Editor, Senior Moderator
Bioengineered. 2019 Dec;10(1):33-42. doi: 10.1080/21655979.2019.1586054.
[h=1]Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus.[/h] Ozkan B[SUP]1[/SUP], Budama-Kilinc Y[SUP]1[/SUP], Cakir-Koc R[SUP]1[/SUP], Mese S[SUP]2[/SUP], Badur S[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescence and Fourier-transform infrared spectroscopy. Subsequently, the diagnostic value of this bioconjugate was evaluated by direct sandwich ELISA using nasopharyngeal swab samples positive/negative for H1N1 and H3N2, which were previously analyzed by rRT-PCR for influenza. In conclusion, the M2e-specific IgY-ALP bioconjugate demonstrated positive results for Influenza A in samples that were diagnosed as Influenza A via the RT-PCR method.
[h=4]KEYWORDS:[/h] ELISA methods; Immunoglobulin Y; alkaline phosphatase; diagnosis; influenza A
PMID: 30913952 DOI: 10.1080/21655979.2019.1586054
[h=1]Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus.[/h] Ozkan B[SUP]1[/SUP], Budama-Kilinc Y[SUP]1[/SUP], Cakir-Koc R[SUP]1[/SUP], Mese S[SUP]2[/SUP], Badur S[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescence and Fourier-transform infrared spectroscopy. Subsequently, the diagnostic value of this bioconjugate was evaluated by direct sandwich ELISA using nasopharyngeal swab samples positive/negative for H1N1 and H3N2, which were previously analyzed by rRT-PCR for influenza. In conclusion, the M2e-specific IgY-ALP bioconjugate demonstrated positive results for Influenza A in samples that were diagnosed as Influenza A via the RT-PCR method.
[h=4]KEYWORDS:[/h] ELISA methods; Immunoglobulin Y; alkaline phosphatase; diagnosis; influenza A
PMID: 30913952 DOI: 10.1080/21655979.2019.1586054