tetano
Editor, Senior Moderator
Arch Med Res
. 2022 Dec 28;S0188-4409(22)00187-4.
doi: 10.1016/j.arcmed.2022.12.010. Online ahead of print.
Impact of TRIM5α and TRIM22 Genes Expression on the Clinical Course of Coronavirus Disease 2019
Rezvan Tavakoli[SUP] 1 [/SUP], Pooneh Rahimi[SUP] 2 [/SUP], Mojtaba Hamidi-Fard[SUP] 2 [/SUP], Sana Eybpoosh[SUP] 3 [/SUP], Delaram Doroud[SUP] 4 [/SUP], Seyed Amir Sadeghi[SUP] 1 [/SUP], Mohammadali Zaheri Birgani[SUP] 1 [/SUP], Mohammadreza Aghasadeghi[SUP] 5 [/SUP], Abolfazl Fateh[SUP] 6 [/SUP]
Affiliations
Abstract
Objective: The innate immune response in humans involves a wide variety of factors, including the tripartite motif-containing 5α (TRIM5α) and 22 (TRIM22) as a cluster of genes on chromosome 11 that have exhibited antiviral activity in several viral infections. We analyzed the correlation of the expression of TRIM5α and TRIM22 with the severity of Coronavirus Disease 2019 (COVID-19) in blood samples of 330 patients, divided into two groups of severe and mild disease, versus the healthy individuals who never had contact with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
Methods: The transcription level of TRIM5α and TRIM22 was determined by quantitative real-time polymerase chain reaction (qPCR). The laboratory values were collected from the patients' records.
Results: The expression of both genes was significantly lower in the severe group containing the hospitalized patients than in both the mild group and the control group. However, in the mild group, TRIM22 expression was significantly higher (p <0.0001) than in the control group while TRIM5α expression was not significantly different between these two groups. We found a relationship between the cycle threshold (Ct) value of patients and the expression of the aforementioned genes.
Conclusion: The results of our study indicated that lower Ct values or higher RNA viral load might be associated with the downregulation of TRIM5α and TRIM22 and the severity of COVID-19. Additional studies are needed to confirm the results of this study.
Keywords: Coronavirus disease 2019; Tripartite motif-containing 22; Tripartite motif-containing 5α; cycle threshold value; mild infection; severe infection.
. 2022 Dec 28;S0188-4409(22)00187-4.
doi: 10.1016/j.arcmed.2022.12.010. Online ahead of print.
Impact of TRIM5α and TRIM22 Genes Expression on the Clinical Course of Coronavirus Disease 2019
Rezvan Tavakoli[SUP] 1 [/SUP], Pooneh Rahimi[SUP] 2 [/SUP], Mojtaba Hamidi-Fard[SUP] 2 [/SUP], Sana Eybpoosh[SUP] 3 [/SUP], Delaram Doroud[SUP] 4 [/SUP], Seyed Amir Sadeghi[SUP] 1 [/SUP], Mohammadali Zaheri Birgani[SUP] 1 [/SUP], Mohammadreza Aghasadeghi[SUP] 5 [/SUP], Abolfazl Fateh[SUP] 6 [/SUP]
Affiliations
- PMID: 36621405
- DOI: 10.1016/j.arcmed.2022.12.010
Abstract
Objective: The innate immune response in humans involves a wide variety of factors, including the tripartite motif-containing 5α (TRIM5α) and 22 (TRIM22) as a cluster of genes on chromosome 11 that have exhibited antiviral activity in several viral infections. We analyzed the correlation of the expression of TRIM5α and TRIM22 with the severity of Coronavirus Disease 2019 (COVID-19) in blood samples of 330 patients, divided into two groups of severe and mild disease, versus the healthy individuals who never had contact with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
Methods: The transcription level of TRIM5α and TRIM22 was determined by quantitative real-time polymerase chain reaction (qPCR). The laboratory values were collected from the patients' records.
Results: The expression of both genes was significantly lower in the severe group containing the hospitalized patients than in both the mild group and the control group. However, in the mild group, TRIM22 expression was significantly higher (p <0.0001) than in the control group while TRIM5α expression was not significantly different between these two groups. We found a relationship between the cycle threshold (Ct) value of patients and the expression of the aforementioned genes.
Conclusion: The results of our study indicated that lower Ct values or higher RNA viral load might be associated with the downregulation of TRIM5α and TRIM22 and the severity of COVID-19. Additional studies are needed to confirm the results of this study.
Keywords: Coronavirus disease 2019; Tripartite motif-containing 22; Tripartite motif-containing 5α; cycle threshold value; mild infection; severe infection.