tetano
Editor, Senior Moderator
J Virol. 2014 Jan 8. [Epub ahead of print]
Assessment of influenza virus hemagglutinin stalk-based immunity in ferrets.
Krammer F, Hai R, Yondola M, Tan GS, Leyva-Grado V, Ryder AB, Miller MS, Rose JK, Palese P, Garc?a-Sastre A, Albrecht RA.
Author information
Abstract
Therapeutic monoclonal antibodies that target the conserved stalk domain of the influenza virus hemagglutinin as well as stalk-based universal influenza virus vaccine strategies are being developed as promising countermeasures for influenza virus infections. The pan-H1 reactive monoclonal antibody 6F12 has been extensively characterized and shows broad efficacy against divergent H1N1 strains in the mouse model. Here we demonstrate its efficacy against a pandemic H1N1 challenge virus in the ferret model of influenza disease. Furthermore, we recently developed a universal influenza virus vaccine strategy based on chimeric hemagglutinin constructs that focuses the immune response towards the conserved stalk domain of the hemagglutinin. Here we set out to test this vaccination strategy in the ferret model. Both strategies, pre-treatment of animals with stalk-reactive monoclonal antibody as well as vaccination with chimeric hemagglutinin based constructs were able to significantly reduce viral titers in nasal turbinates, lungs and olfactory bulbs. In addition, vaccinated animals also showed reduced nasal wash viral titers. In summary both strategies showed efficacy in reducing viral loads after influenza virus challenge in the ferret model.
PMID:
24403585
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24403585
Assessment of influenza virus hemagglutinin stalk-based immunity in ferrets.
Krammer F, Hai R, Yondola M, Tan GS, Leyva-Grado V, Ryder AB, Miller MS, Rose JK, Palese P, Garc?a-Sastre A, Albrecht RA.
Author information
Abstract
Therapeutic monoclonal antibodies that target the conserved stalk domain of the influenza virus hemagglutinin as well as stalk-based universal influenza virus vaccine strategies are being developed as promising countermeasures for influenza virus infections. The pan-H1 reactive monoclonal antibody 6F12 has been extensively characterized and shows broad efficacy against divergent H1N1 strains in the mouse model. Here we demonstrate its efficacy against a pandemic H1N1 challenge virus in the ferret model of influenza disease. Furthermore, we recently developed a universal influenza virus vaccine strategy based on chimeric hemagglutinin constructs that focuses the immune response towards the conserved stalk domain of the hemagglutinin. Here we set out to test this vaccination strategy in the ferret model. Both strategies, pre-treatment of animals with stalk-reactive monoclonal antibody as well as vaccination with chimeric hemagglutinin based constructs were able to significantly reduce viral titers in nasal turbinates, lungs and olfactory bulbs. In addition, vaccinated animals also showed reduced nasal wash viral titers. In summary both strategies showed efficacy in reducing viral loads after influenza virus challenge in the ferret model.
PMID:
24403585
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24403585