tetano
Editor, Senior Moderator
Int J Infect Dis. 2015 Jul 7. pii: S1201-9712(15)00169-1. doi: 10.1016/j.ijid.2015.07.002. [Epub ahead of print]
[h=1]Attenuation of the influenza virus by microRNA response element in vivo and protective efficacy against 2009 pandemic H1N1 Virus in mice.[/h] Feng C[SUP]1[/SUP], Tan M[SUP]1[/SUP], Sun W[SUP]1[/SUP], Shi Y[SUP]2[/SUP], Xing Z[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The 2009 influenza pandemics underscored the need for effective vaccines to block the spread of influenza virus infection. Most live attenuated vaccines utilize cold-adapted, temperature-sensitive virus. Here, we present an alternative to live attenuated virus, and is based on microRNA-induced gene silencing.
[h=4]METHODS:[/h] In this study, we inserted miR-let-7b target sequences into H1N1 genome to engineer a recombinant virus miRT-H1N1. Female BALB/c mice were intranasally vaccinated with the miRT-H1N1 and challenged with a lethal dose of homologous virus.
[h=4]RESULTS:[/h] This miRT-H1N1 virus was attenuated in mice while it exhibited wild-type characteristics in chicken embryos. Mice intranasally vaccinated with the miRT-H1N1 responded with robust immunity that protected the vaccinated mice from a lethal challenge the wild-type 2009 pandemic H1N1 virus.
[h=4]CONCLUSIONS:[/h] Our results indicate that the influenza virus containing microRNA response elements (MREs) is attenuated in vivo and can be used to design a live attenuated vaccine.
Copyright ? 2015. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] H1N1; Immunogenicity; Influenza; Live attenuated vaccine
PMID: 26163223 [PubMed - as supplied by publisher] Free full text
[h=1]Attenuation of the influenza virus by microRNA response element in vivo and protective efficacy against 2009 pandemic H1N1 Virus in mice.[/h] Feng C[SUP]1[/SUP], Tan M[SUP]1[/SUP], Sun W[SUP]1[/SUP], Shi Y[SUP]2[/SUP], Xing Z[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The 2009 influenza pandemics underscored the need for effective vaccines to block the spread of influenza virus infection. Most live attenuated vaccines utilize cold-adapted, temperature-sensitive virus. Here, we present an alternative to live attenuated virus, and is based on microRNA-induced gene silencing.
[h=4]METHODS:[/h] In this study, we inserted miR-let-7b target sequences into H1N1 genome to engineer a recombinant virus miRT-H1N1. Female BALB/c mice were intranasally vaccinated with the miRT-H1N1 and challenged with a lethal dose of homologous virus.
[h=4]RESULTS:[/h] This miRT-H1N1 virus was attenuated in mice while it exhibited wild-type characteristics in chicken embryos. Mice intranasally vaccinated with the miRT-H1N1 responded with robust immunity that protected the vaccinated mice from a lethal challenge the wild-type 2009 pandemic H1N1 virus.
[h=4]CONCLUSIONS:[/h] Our results indicate that the influenza virus containing microRNA response elements (MREs) is attenuated in vivo and can be used to design a live attenuated vaccine.
Copyright ? 2015. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] H1N1; Immunogenicity; Influenza; Live attenuated vaccine
PMID: 26163223 [PubMed - as supplied by publisher] Free full text