tetano
Editor, Senior Moderator
Biochem Biophys Res Commun
. 2020 Nov 20;S0006-291X(20)32104-5.
doi: 10.1016/j.bbrc.2020.11.060. Online ahead of print.
Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
Airi Rump[SUP] 1 [/SUP], Robert Risti[SUP] 2 [/SUP], Mai-Ly Kristal[SUP] 3 [/SUP], Jekaterina Reut[SUP] 4 [/SUP], Vitali Syritski[SUP] 5 [/SUP], Aivar Lookene[SUP] 6 [/SUP], Sirje Ruutel Boudinot[SUP] 7 [/SUP]
Affiliations
Abstract
Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-negative serological tests. Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes.
Keywords: Glycosylation; Nucleocapsid protein (NP); SARS-CoV-2.
. 2020 Nov 20;S0006-291X(20)32104-5.
doi: 10.1016/j.bbrc.2020.11.060. Online ahead of print.
Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
Airi Rump[SUP] 1 [/SUP], Robert Risti[SUP] 2 [/SUP], Mai-Ly Kristal[SUP] 3 [/SUP], Jekaterina Reut[SUP] 4 [/SUP], Vitali Syritski[SUP] 5 [/SUP], Aivar Lookene[SUP] 6 [/SUP], Sirje Ruutel Boudinot[SUP] 7 [/SUP]
Affiliations
- PMID: 33276951
- DOI: 10.1016/j.bbrc.2020.11.060
Abstract
Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-negative serological tests. Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes.
Keywords: Glycosylation; Nucleocapsid protein (NP); SARS-CoV-2.