tetano
Editor, Senior Moderator
Bioelectrochemistry
. 2024 Feb 25:157:108672.
doi: 10.1016/j.bioelechem.2024.108672. Online ahead of print. Photo-sensitive peptide inducing targeted cross-linking in a one-step and reagent-, enzyme- and antibody-free detection of SARS-Cov-2 marker protein
Meihui Hou[SUP] 1 [/SUP], Wenmin Hou[SUP] 1 [/SUP], Mingyu Qin[SUP] 2 [/SUP], Qun Wang[SUP] 3 [/SUP], Lei Zhou[SUP] 4 [/SUP]
Affiliations
Modern biosensing technology plays a crucial role in combating the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). However, the associated assays remain costly, considering their extensive daily use. In response, we developed a simplified one-step SARS-CoV-2 protease assay that reduces both time and financial expenses. This approach eliminates the need for extra reagents, enzymes, or antibodies. The simplification involves a photo-sensitive Bengal red-tagged substrate peptide, allowing specific cross-linking upon protease-substrate recognition. This process forms a di-tyrosine product with a distinctive fluorescence signal readout, enabling the detection of SARS-CoV-2 in patient serum samples. This method anticipates a major reduction in assay costs in the near future.
Keywords: COVID-2019; Covalent biosensing; Peptide; Photo-induced cross-linking; Proximity-induce labeling; SARS-Cov-2.
. 2024 Feb 25:157:108672.
doi: 10.1016/j.bioelechem.2024.108672. Online ahead of print. Photo-sensitive peptide inducing targeted cross-linking in a one-step and reagent-, enzyme- and antibody-free detection of SARS-Cov-2 marker protein
Meihui Hou[SUP] 1 [/SUP], Wenmin Hou[SUP] 1 [/SUP], Mingyu Qin[SUP] 2 [/SUP], Qun Wang[SUP] 3 [/SUP], Lei Zhou[SUP] 4 [/SUP]
Affiliations
- PMID: 38428185
- DOI: 10.1016/j.bioelechem.2024.108672
Modern biosensing technology plays a crucial role in combating the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). However, the associated assays remain costly, considering their extensive daily use. In response, we developed a simplified one-step SARS-CoV-2 protease assay that reduces both time and financial expenses. This approach eliminates the need for extra reagents, enzymes, or antibodies. The simplification involves a photo-sensitive Bengal red-tagged substrate peptide, allowing specific cross-linking upon protease-substrate recognition. This process forms a di-tyrosine product with a distinctive fluorescence signal readout, enabling the detection of SARS-CoV-2 in patient serum samples. This method anticipates a major reduction in assay costs in the near future.
Keywords: COVID-2019; Covalent biosensing; Peptide; Photo-induced cross-linking; Proximity-induce labeling; SARS-Cov-2.