tetano
Editor, Senior Moderator
Biomed Environ Sci
. 2023 Mar 20;36(3):269-278.
doi: 10.3967/bes2023.029.
Highly Sensitive Poly-N-isopropylacrylamide Microgel-based Electrochemical Biosensor for the Detection of SARS-COV-2 Spike Protein
Hao Chen[SUP] 1 [/SUP], Zhi Yuan Hou[SUP] 2 [/SUP], Die Chen[SUP] 2 [/SUP], Ting Li[SUP] 2 [/SUP], Yi Ming Wang[SUP] 3 [/SUP], Marcelo Andrade De Lima[SUP] 4 [/SUP], Ying Yang[SUP] 5 [/SUP], Zhen Zhong Guo[SUP] 6 [/SUP]
Affiliations
Abstract
Objective: Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.
Methods: In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.
Results: Under optimal experimental conditions, the linear range of the sensor was 10 [SUP]-13[/SUP]-10 [SUP]-9[/SUP] mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability.
Conclusion: The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.
Keywords: Detection; Microgel; SARS-CoV-2; Spike protein.
. 2023 Mar 20;36(3):269-278.
doi: 10.3967/bes2023.029.
Highly Sensitive Poly-N-isopropylacrylamide Microgel-based Electrochemical Biosensor for the Detection of SARS-COV-2 Spike Protein
Hao Chen[SUP] 1 [/SUP], Zhi Yuan Hou[SUP] 2 [/SUP], Die Chen[SUP] 2 [/SUP], Ting Li[SUP] 2 [/SUP], Yi Ming Wang[SUP] 3 [/SUP], Marcelo Andrade De Lima[SUP] 4 [/SUP], Ying Yang[SUP] 5 [/SUP], Zhen Zhong Guo[SUP] 6 [/SUP]
Affiliations
- PMID: 37005080
- DOI: 10.3967/bes2023.029
Abstract
Objective: Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.
Methods: In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.
Results: Under optimal experimental conditions, the linear range of the sensor was 10 [SUP]-13[/SUP]-10 [SUP]-9[/SUP] mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability.
Conclusion: The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.
Keywords: Detection; Microgel; SARS-CoV-2; Spike protein.