tetano
Editor, Senior Moderator
Biomol Ther (Seoul)
. 2021 Jan 28.
doi: 10.4062/biomolther.2020.226. Online ahead of print.
Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells upon SARS-CoV-2 Infection
Byoung Kwon Park[SUP] 1 [/SUP], Dongbum Kim[SUP] 1 [/SUP], Sangkyu Park[SUP] 2 [/SUP], Sony Maharjan[SUP] 1 [/SUP], Jinsoo Kim[SUP] 3 [/SUP], Jun-Kyu Choi[SUP] 2 [/SUP], Madhav Akauliya[SUP] 3 [/SUP], Younghee Lee[SUP] 2 [/SUP], Hyung-Joo Kwon[SUP] 1 3 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is responsible for the current coronavirus disease 2019 (COVID-19) pandemic. Signaling pathways that are essential for virus production have potential as therapeutic targets against COVID-19. In this study, we investigated cellular responses in two cell lines, Vero and Calu-3, upon SARS-CoV-2 infection and evaluated the effects of pathway-specific inhibitors on virus production. SARS-CoV-2 infection induced dephosphorylation of STAT1 and STAT3, high virus production, and apoptosis in Vero cells. However, in Calu-3 cells, SARS-CoV-2 infection induced long-lasting phosphorylation of STAT1 and STAT3, low virus production, and no prominent apoptosis. Inhibitors that target STAT3 phosphorylation and dimerization reduced SARS-CoV-2 production in Calu-3 cells, but not in Vero cells. These results suggest a necessity to evaluate cellular consequences upon SARS-CoV-2 infection using various model cell lines to find out more appropriate cells recapitulating relevant responses to SARS-CoV-2 infection in vitro.
Keywords: Apoptosis; COVID-19; SARS-CoV-2; STAT1; STAT3; STAT3 phosphorylation.
. 2021 Jan 28.
doi: 10.4062/biomolther.2020.226. Online ahead of print.
Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells upon SARS-CoV-2 Infection
Byoung Kwon Park[SUP] 1 [/SUP], Dongbum Kim[SUP] 1 [/SUP], Sangkyu Park[SUP] 2 [/SUP], Sony Maharjan[SUP] 1 [/SUP], Jinsoo Kim[SUP] 3 [/SUP], Jun-Kyu Choi[SUP] 2 [/SUP], Madhav Akauliya[SUP] 3 [/SUP], Younghee Lee[SUP] 2 [/SUP], Hyung-Joo Kwon[SUP] 1 3 [/SUP]
Affiliations
- PMID: 33504682
- DOI: 10.4062/biomolther.2020.226
Abstract
Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is responsible for the current coronavirus disease 2019 (COVID-19) pandemic. Signaling pathways that are essential for virus production have potential as therapeutic targets against COVID-19. In this study, we investigated cellular responses in two cell lines, Vero and Calu-3, upon SARS-CoV-2 infection and evaluated the effects of pathway-specific inhibitors on virus production. SARS-CoV-2 infection induced dephosphorylation of STAT1 and STAT3, high virus production, and apoptosis in Vero cells. However, in Calu-3 cells, SARS-CoV-2 infection induced long-lasting phosphorylation of STAT1 and STAT3, low virus production, and no prominent apoptosis. Inhibitors that target STAT3 phosphorylation and dimerization reduced SARS-CoV-2 production in Calu-3 cells, but not in Vero cells. These results suggest a necessity to evaluate cellular consequences upon SARS-CoV-2 infection using various model cell lines to find out more appropriate cells recapitulating relevant responses to SARS-CoV-2 infection in vitro.
Keywords: Apoptosis; COVID-19; SARS-CoV-2; STAT1; STAT3; STAT3 phosphorylation.