tetano
Editor, Senior Moderator
Biosci Biotechnol Biochem
. 2023 Mar 30;zbad037.
doi: 10.1093/bbb/zbad037. Online ahead of print.
An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms
Izza Nur Laily[SUP] 1 2 [/SUP], Michiki Takeuchi[SUP] 3 [/SUP], Taku Mizutani[SUP] 1 [/SUP], Jun Ogawa[SUP] 1 [/SUP]
Affiliations
Abstract
Angiotensin-converting enzyme 2 (ACE2) is a binding target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. An ACE2-like enzyme, such as bacterial M32-carboxypeptidase (M32-CAP), is assumed to be a potential therapeutic candidate for coronavirus disease 2019 (COVID-19). Here, we screened bacteria with an ACE2-like enzyme activity from Japanese fermented food and dietary products using the fluorogenic substrate for rapid screening. The strain showing the highest activity, Enterobacter sp. 200527-13, produced an enzyme with the same hydrolytic activity as ACE2 on Angiotensin II (Ang II). The enzymatic analysis using the heterologously-expressed enzyme in E. coli revealed that the enzyme catalyzes the same reaction with that of ACE2, Ang II hydrolysis to Ang 1-7 and phenylalanine. The gene sequence information showed that the enzyme belongs to the M32-CAP family. These results suggested that the selected enzyme, M32-CAP (EntCP), from Enterobacter sp. 200527-13 was identified as an ACE2-like enzyme.
Keywords: Enterobacter sp; ACE2; M32-carboxypeptidase; SARS-CoV-2.
. 2023 Mar 30;zbad037.
doi: 10.1093/bbb/zbad037. Online ahead of print.
An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms
Izza Nur Laily[SUP] 1 2 [/SUP], Michiki Takeuchi[SUP] 3 [/SUP], Taku Mizutani[SUP] 1 [/SUP], Jun Ogawa[SUP] 1 [/SUP]
Affiliations
- PMID: 36997336
- DOI: 10.1093/bbb/zbad037
Abstract
Angiotensin-converting enzyme 2 (ACE2) is a binding target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. An ACE2-like enzyme, such as bacterial M32-carboxypeptidase (M32-CAP), is assumed to be a potential therapeutic candidate for coronavirus disease 2019 (COVID-19). Here, we screened bacteria with an ACE2-like enzyme activity from Japanese fermented food and dietary products using the fluorogenic substrate for rapid screening. The strain showing the highest activity, Enterobacter sp. 200527-13, produced an enzyme with the same hydrolytic activity as ACE2 on Angiotensin II (Ang II). The enzymatic analysis using the heterologously-expressed enzyme in E. coli revealed that the enzyme catalyzes the same reaction with that of ACE2, Ang II hydrolysis to Ang 1-7 and phenylalanine. The gene sequence information showed that the enzyme belongs to the M32-CAP family. These results suggested that the selected enzyme, M32-CAP (EntCP), from Enterobacter sp. 200527-13 was identified as an ACE2-like enzyme.
Keywords: Enterobacter sp; ACE2; M32-carboxypeptidase; SARS-CoV-2.