tetano
Editor, Senior Moderator
Biosens Bioelectron
. 2020 Sep 21;169:112643.
doi: 10.1016/j.bios.2020.112643. Online ahead of print.
Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
Daniel J Steiner[SUP] 1 [/SUP], John S Cognetti[SUP] 2 [/SUP], Ethan P Luta[SUP] 3 [/SUP], Alanna M Klose[SUP] 3 [/SUP], Joseph Bucukovski[SUP] 1 [/SUP], Michael R Bryan[SUP] 1 [/SUP], Jon J Schmuke[SUP] 4 [/SUP], Phuong Nguyen-Contant[SUP] 5 [/SUP], Mark Y Sangster[SUP] 5 [/SUP], David J Topham[SUP] 5 [/SUP], Benjamin L Miller[SUP] 6 [/SUP]
Affiliations
Abstract
Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free antigen microarray on the Arrayed Imaging Reflectometry (AIR) platform for detection of antibodies to SARS-CoV-2, SARS-CoV-1, MERS, three circulating coronavirus strains (HKU1, 229E, OC43) and three strains of influenza. We find that the array is readily able to distinguish uninfected from convalescent COVID-19 subjects, and provides quantitative information about total Ig, as well as IgG- and IgM-specific responses.
Keywords: Antigen microarray; Coronavirus; Influenza virus; Label-free biosensor; SARS-CoV-2.
. 2020 Sep 21;169:112643.
doi: 10.1016/j.bios.2020.112643. Online ahead of print.
Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
Daniel J Steiner[SUP] 1 [/SUP], John S Cognetti[SUP] 2 [/SUP], Ethan P Luta[SUP] 3 [/SUP], Alanna M Klose[SUP] 3 [/SUP], Joseph Bucukovski[SUP] 1 [/SUP], Michael R Bryan[SUP] 1 [/SUP], Jon J Schmuke[SUP] 4 [/SUP], Phuong Nguyen-Contant[SUP] 5 [/SUP], Mark Y Sangster[SUP] 5 [/SUP], David J Topham[SUP] 5 [/SUP], Benjamin L Miller[SUP] 6 [/SUP]
Affiliations
- PMID: 33007615
- DOI: 10.1016/j.bios.2020.112643
Abstract
Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free antigen microarray on the Arrayed Imaging Reflectometry (AIR) platform for detection of antibodies to SARS-CoV-2, SARS-CoV-1, MERS, three circulating coronavirus strains (HKU1, 229E, OC43) and three strains of influenza. We find that the array is readily able to distinguish uninfected from convalescent COVID-19 subjects, and provides quantitative information about total Ig, as well as IgG- and IgM-specific responses.
Keywords: Antigen microarray; Coronavirus; Influenza virus; Label-free biosensor; SARS-CoV-2.