tetano
Editor, Senior Moderator
Biosens Bioelectron
. 2021 Jan 23;178:113029.
doi: 10.1016/j.bios.2021.113029. Online ahead of print.
Development of a portable MIP-based electrochemical sensor for detection of SARS-CoV-2 antigen
Abdul Raziq[SUP] 1 [/SUP], Anna Kidakova[SUP] 1 [/SUP], Roman Boroznjak[SUP] 1 [/SUP], Jekaterina Reut[SUP] 1 [/SUP], Andres ?pik[SUP] 1 [/SUP], Vitali Syritski[SUP] 2 [/SUP]
Affiliations
Abstract
The current COVID-19 pandemic caused by SARS-CoV-2 coronavirus is expanding around the globe. Hence, accurate and cheap portable sensors are crucially important for the clinical diagnosis of COVID-19. Molecularly imprinted polymers (MIPs) as robust synthetic molecular recognition materials with antibody-like ability to bind and discriminate between molecules can perfectly serve in building selective elements in such sensors. Herein, we report for the first time on the development of a MIP-based electrochemical sensor for detection of SARS-CoV-2 nucleoprotein (ncovNP). A key element of the sensor is a disposable sensor chip - thin film electrode - interfaced with a MIP-endowed selectivity for ncovNP and connected with a portable potentiostat. The resulting ncovNP sensor showed a linear response to ncovNP in the lysis buffer up to 111 fM with a detection and quantification limit of 15 fM and 50 fM, respectively. Notably, the sensor was capable of signaling ncovNP presence in nasopharyngeal swab samples of COVID-19 positive patients. The presented strategy unlocks a new route for the development of rapid COVID-19 diagnostic tools.
Keywords: COVID-19 antigen test; Electrochemical sensor; Molecularly imprinted polymer; SARS-CoV-2 nucleoprotein.
. 2021 Jan 23;178:113029.
doi: 10.1016/j.bios.2021.113029. Online ahead of print.
Development of a portable MIP-based electrochemical sensor for detection of SARS-CoV-2 antigen
Abdul Raziq[SUP] 1 [/SUP], Anna Kidakova[SUP] 1 [/SUP], Roman Boroznjak[SUP] 1 [/SUP], Jekaterina Reut[SUP] 1 [/SUP], Andres ?pik[SUP] 1 [/SUP], Vitali Syritski[SUP] 2 [/SUP]
Affiliations
- PMID: 33515985
- DOI: 10.1016/j.bios.2021.113029
Abstract
The current COVID-19 pandemic caused by SARS-CoV-2 coronavirus is expanding around the globe. Hence, accurate and cheap portable sensors are crucially important for the clinical diagnosis of COVID-19. Molecularly imprinted polymers (MIPs) as robust synthetic molecular recognition materials with antibody-like ability to bind and discriminate between molecules can perfectly serve in building selective elements in such sensors. Herein, we report for the first time on the development of a MIP-based electrochemical sensor for detection of SARS-CoV-2 nucleoprotein (ncovNP). A key element of the sensor is a disposable sensor chip - thin film electrode - interfaced with a MIP-endowed selectivity for ncovNP and connected with a portable potentiostat. The resulting ncovNP sensor showed a linear response to ncovNP in the lysis buffer up to 111 fM with a detection and quantification limit of 15 fM and 50 fM, respectively. Notably, the sensor was capable of signaling ncovNP presence in nasopharyngeal swab samples of COVID-19 positive patients. The presented strategy unlocks a new route for the development of rapid COVID-19 diagnostic tools.
Keywords: COVID-19 antigen test; Electrochemical sensor; Molecularly imprinted polymer; SARS-CoV-2 nucleoprotein.