tetano
Editor, Senior Moderator
J Immunol Methods. 2018 Jul 24. pii: S0022-1759(17)30448-9. doi: 10.1016/j.jim.2018.07.007. [Epub ahead of print]
[h=1]Biosensor-based epitope mapping of antibodies targeting the hemagglutinin and neuraminidase of influenza A virus.[/h] Guo Z[SUP]1[/SUP], Wilson JR[SUP]2[/SUP], York IA[SUP]3[/SUP], Stevens J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Characterization of the epitopes on antigen recognized by monoclonal antibodies (mAb) is useful for the development of therapeutic antibodies, diagnostic tools, and vaccines. Epitope mapping also provides functional information for sequence-based repertoire analysis of antibody response to pathogen infection and/or vaccination. However, development of mapping strategies has lagged behind mAb discovery. We have developed a site-directed mutagenesis approach that can be used in conjunction with bio-layer interferometry (BLI) biosensors to map mAb epitopes. By generating a panel of single point mutants in the recombinant hemagglutinin (HA) and neuraminidase (NA) proteins of influenza A viruses, we have characterized the epitopes of hundreds of mAbs targeting the H1 and H3 subtypes of HA and the N9 subtype of NA.
PMID: 30053389 DOI: 10.1016/j.jim.2018.07.007
[h=1]Biosensor-based epitope mapping of antibodies targeting the hemagglutinin and neuraminidase of influenza A virus.[/h] Guo Z[SUP]1[/SUP], Wilson JR[SUP]2[/SUP], York IA[SUP]3[/SUP], Stevens J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Characterization of the epitopes on antigen recognized by monoclonal antibodies (mAb) is useful for the development of therapeutic antibodies, diagnostic tools, and vaccines. Epitope mapping also provides functional information for sequence-based repertoire analysis of antibody response to pathogen infection and/or vaccination. However, development of mapping strategies has lagged behind mAb discovery. We have developed a site-directed mutagenesis approach that can be used in conjunction with bio-layer interferometry (BLI) biosensors to map mAb epitopes. By generating a panel of single point mutants in the recombinant hemagglutinin (HA) and neuraminidase (NA) proteins of influenza A viruses, we have characterized the epitopes of hundreds of mAbs targeting the H1 and H3 subtypes of HA and the N9 subtype of NA.
PMID: 30053389 DOI: 10.1016/j.jim.2018.07.007