tetano
Editor, Senior Moderator
BMC Infect Dis
. 2022 Oct 13;22(1):785.
doi: 10.1186/s12879-022-07766-9.
Diagnostic accuracy of multiplex respiratory pathogen panels for influenza or respiratory syncytial virus infections: systematic review and meta-analysis
Sophie Jullien[SUP] #[/SUP][SUP] 1 [/SUP], Felicity Fitzgerald[SUP] #[/SUP][SUP] 2 [/SUP], Suzanne Keddie[SUP] #[/SUP][SUP] 3 [/SUP], Oliver Baerenbold[SUP] 2 [/SUP], Quique Bassat[SUP] 4 5 6 7 8 [/SUP], John Bradley[SUP] 3 [/SUP], Jane Falconer[SUP] 3 [/SUP], Colin Fink[SUP] 9 10 [/SUP], Ruth Keogh[SUP] 3 [/SUP], Heidi Hopkins[SUP] #[/SUP][SUP] 3 [/SUP], Marie Voice[SUP] #[/SUP][SUP] 9 [/SUP]
Affiliations
Abstract
Respiratory syncytial virus (RSV) and influenza viruses are important global causes of morbidity and mortality. We evaluated the diagnostic accuracy of the Luminex NxTAG respiratory pathogen panels (RPPs)™ (index) against other RPPs (comparator) for detection of RSV and influenza viruses. Studies comparing human clinical respiratory samples tested with the index and at least one comparator test were included. A random-effect latent class meta-analysis was performed to assess the specificity and sensitivity of the index test for RSV and influenza. Risk of bias was assessed using the QUADAS-2 tool and certainty of evidence using GRADE. Ten studies were included. For RSV, predicted sensitivity was 99% (95% credible interval [CrI] 96-100%) and specificity 100% (95% CrI 98-100%). For influenza A and B, predicted sensitivity was 97% (95% CrI 89-100) and 98% (95% CrI 88-100) respectively; specificity 100% (95% CrI 99-100) and 100% (95% CrI 99-100), respectively. Evidence was low certainty. Although index sensitivity and specificity were excellent, comparators' performance varied. Further research with clear patient recruitment strategies could ascertain performance across different populations.Protocol Registration: Prospero CRD42021272062.
Keywords: Diagnosis; Influenza virus; Molecular diagnostics; Respiratory infection; Respiratory syncytial virus.
. 2022 Oct 13;22(1):785.
doi: 10.1186/s12879-022-07766-9.
Diagnostic accuracy of multiplex respiratory pathogen panels for influenza or respiratory syncytial virus infections: systematic review and meta-analysis
Sophie Jullien[SUP] #[/SUP][SUP] 1 [/SUP], Felicity Fitzgerald[SUP] #[/SUP][SUP] 2 [/SUP], Suzanne Keddie[SUP] #[/SUP][SUP] 3 [/SUP], Oliver Baerenbold[SUP] 2 [/SUP], Quique Bassat[SUP] 4 5 6 7 8 [/SUP], John Bradley[SUP] 3 [/SUP], Jane Falconer[SUP] 3 [/SUP], Colin Fink[SUP] 9 10 [/SUP], Ruth Keogh[SUP] 3 [/SUP], Heidi Hopkins[SUP] #[/SUP][SUP] 3 [/SUP], Marie Voice[SUP] #[/SUP][SUP] 9 [/SUP]
Affiliations
- PMID: 36229786
- DOI: 10.1186/s12879-022-07766-9
Abstract
Respiratory syncytial virus (RSV) and influenza viruses are important global causes of morbidity and mortality. We evaluated the diagnostic accuracy of the Luminex NxTAG respiratory pathogen panels (RPPs)™ (index) against other RPPs (comparator) for detection of RSV and influenza viruses. Studies comparing human clinical respiratory samples tested with the index and at least one comparator test were included. A random-effect latent class meta-analysis was performed to assess the specificity and sensitivity of the index test for RSV and influenza. Risk of bias was assessed using the QUADAS-2 tool and certainty of evidence using GRADE. Ten studies were included. For RSV, predicted sensitivity was 99% (95% credible interval [CrI] 96-100%) and specificity 100% (95% CrI 98-100%). For influenza A and B, predicted sensitivity was 97% (95% CrI 89-100) and 98% (95% CrI 88-100) respectively; specificity 100% (95% CrI 99-100) and 100% (95% CrI 99-100), respectively. Evidence was low certainty. Although index sensitivity and specificity were excellent, comparators' performance varied. Further research with clear patient recruitment strategies could ascertain performance across different populations.Protocol Registration: Prospero CRD42021272062.
Keywords: Diagnosis; Influenza virus; Molecular diagnostics; Respiratory infection; Respiratory syncytial virus.