tetano
Editor, Senior Moderator
Cell
. 2020 Aug 5;S0092-8674(20)30993-4.
doi: 10.1016/j.cell.2020.08.002. Online ahead of print.
Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19
Aymeric Silvin[SUP] 1 [/SUP], Nicolas Chapuis[SUP] 2 [/SUP], Garett Dunsmore[SUP] 1 [/SUP], Anne-Ga?lle Goubet[SUP] 1 [/SUP], Agathe Dubuisson[SUP] 1 [/SUP], Lisa Derosa[SUP] 3 [/SUP], Carole Almire[SUP] 4 [/SUP], Cl?mence H?non[SUP] 5 [/SUP], Olivier Kosmider[SUP] 2 [/SUP], Nathalie Droin[SUP] 6 [/SUP], Philippe Rameau[SUP] 7 [/SUP], Cyril Catelain[SUP] 7 [/SUP], Alexia Alfaro[SUP] 7 [/SUP], Charles Dussiau[SUP] 2 [/SUP], Chlo? Friedrich[SUP] 2 [/SUP], Elise Sourdeau[SUP] 8 [/SUP], Nathalie Marin[SUP] 9 [/SUP], Tali-Anne Szwebel[SUP] 10 [/SUP], Delphine Cantin[SUP] 8 [/SUP], Luc Mouthon[SUP] 11 [/SUP], Didier Borderie[SUP] 12 [/SUP], Marc Deloger[SUP] 7 [/SUP], Delphine Bredel[SUP] 1 [/SUP], Severine Mouraud[SUP] 1 [/SUP], Damien Drubay[SUP] 13 [/SUP], Muriel Andrieu[SUP] 14 [/SUP], Anne-Sophie Lhonneur[SUP] 15 [/SUP], V?ronique Saada[SUP] 16 [/SUP], Annabelle Stoclin[SUP] 17 [/SUP], Christophe Willekens[SUP] 18 [/SUP], Fanny Pommeret[SUP] 19 [/SUP], Frank Griscelli[SUP] 20 [/SUP], Lai Guan Ng[SUP] 21 [/SUP], Zheng Zhang[SUP] 22 [/SUP], Pierre Bost[SUP] 23 [/SUP], Ido Amit[SUP] 24 [/SUP], Fabrice Barlesi[SUP] 19 [/SUP], Aur?lien Marabelle[SUP] 25 [/SUP], Fr?d?ric P?ne[SUP] 26 [/SUP], Bertrand Gachot[SUP] 17 [/SUP], Fabrice Andr?[SUP] 27 [/SUP], Laurence Zitvogel[SUP] 28 [/SUP], Florent Ginhoux[SUP] 29 [/SUP], Michaela Fontenay[SUP] 30 [/SUP], Eric Solary[SUP] 31 [/SUP]
Affiliations
Abstract
Blood myeloid cells are known to be dysregulated in coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2. It is unknown whether the innate myeloid response differs with disease severity and whether markers of innate immunity discriminate high-risk patients. Thus, we performed high-dimensional flow cytometry and single-cell RNA sequencing of COVID-19 patient peripheral blood cells and detected disappearance of non-classical CD14[SUP]Low[/SUP]CD16[SUP]High[/SUP] monocytes, accumulation of HLA-DR[SUP]Low[/SUP] classical monocytes (Human Leukocyte Antigen - DR isotype), and release of massive amounts of calprotectin (S100A8/S100A9) in severe cases. Immature CD10[SUP]Low[/SUP]CD101[SUP]-[/SUP]CXCR4[SUP]+/-[/SUP] neutrophils with an immunosuppressive profile accumulated in the blood and lungs, suggesting emergency myelopoiesis. Finally, we show that calprotectin plasma level and a routine flow cytometry assay detecting decreased frequencies of non-classical monocytes could discriminate patients who develop a severe form of COVID-19, suggesting a predictive value that deserves prospective evaluation.
Keywords: COVID-19; S100A8; S100A9; SARS-CoV-2; calprotectin; emergency myelopoiesis; monocyte subsets; neutrophils; type I interferon.
. 2020 Aug 5;S0092-8674(20)30993-4.
doi: 10.1016/j.cell.2020.08.002. Online ahead of print.
Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19
Aymeric Silvin[SUP] 1 [/SUP], Nicolas Chapuis[SUP] 2 [/SUP], Garett Dunsmore[SUP] 1 [/SUP], Anne-Ga?lle Goubet[SUP] 1 [/SUP], Agathe Dubuisson[SUP] 1 [/SUP], Lisa Derosa[SUP] 3 [/SUP], Carole Almire[SUP] 4 [/SUP], Cl?mence H?non[SUP] 5 [/SUP], Olivier Kosmider[SUP] 2 [/SUP], Nathalie Droin[SUP] 6 [/SUP], Philippe Rameau[SUP] 7 [/SUP], Cyril Catelain[SUP] 7 [/SUP], Alexia Alfaro[SUP] 7 [/SUP], Charles Dussiau[SUP] 2 [/SUP], Chlo? Friedrich[SUP] 2 [/SUP], Elise Sourdeau[SUP] 8 [/SUP], Nathalie Marin[SUP] 9 [/SUP], Tali-Anne Szwebel[SUP] 10 [/SUP], Delphine Cantin[SUP] 8 [/SUP], Luc Mouthon[SUP] 11 [/SUP], Didier Borderie[SUP] 12 [/SUP], Marc Deloger[SUP] 7 [/SUP], Delphine Bredel[SUP] 1 [/SUP], Severine Mouraud[SUP] 1 [/SUP], Damien Drubay[SUP] 13 [/SUP], Muriel Andrieu[SUP] 14 [/SUP], Anne-Sophie Lhonneur[SUP] 15 [/SUP], V?ronique Saada[SUP] 16 [/SUP], Annabelle Stoclin[SUP] 17 [/SUP], Christophe Willekens[SUP] 18 [/SUP], Fanny Pommeret[SUP] 19 [/SUP], Frank Griscelli[SUP] 20 [/SUP], Lai Guan Ng[SUP] 21 [/SUP], Zheng Zhang[SUP] 22 [/SUP], Pierre Bost[SUP] 23 [/SUP], Ido Amit[SUP] 24 [/SUP], Fabrice Barlesi[SUP] 19 [/SUP], Aur?lien Marabelle[SUP] 25 [/SUP], Fr?d?ric P?ne[SUP] 26 [/SUP], Bertrand Gachot[SUP] 17 [/SUP], Fabrice Andr?[SUP] 27 [/SUP], Laurence Zitvogel[SUP] 28 [/SUP], Florent Ginhoux[SUP] 29 [/SUP], Michaela Fontenay[SUP] 30 [/SUP], Eric Solary[SUP] 31 [/SUP]
Affiliations
- PMID: 32810439
- DOI: 10.1016/j.cell.2020.08.002
Abstract
Blood myeloid cells are known to be dysregulated in coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2. It is unknown whether the innate myeloid response differs with disease severity and whether markers of innate immunity discriminate high-risk patients. Thus, we performed high-dimensional flow cytometry and single-cell RNA sequencing of COVID-19 patient peripheral blood cells and detected disappearance of non-classical CD14[SUP]Low[/SUP]CD16[SUP]High[/SUP] monocytes, accumulation of HLA-DR[SUP]Low[/SUP] classical monocytes (Human Leukocyte Antigen - DR isotype), and release of massive amounts of calprotectin (S100A8/S100A9) in severe cases. Immature CD10[SUP]Low[/SUP]CD101[SUP]-[/SUP]CXCR4[SUP]+/-[/SUP] neutrophils with an immunosuppressive profile accumulated in the blood and lungs, suggesting emergency myelopoiesis. Finally, we show that calprotectin plasma level and a routine flow cytometry assay detecting decreased frequencies of non-classical monocytes could discriminate patients who develop a severe form of COVID-19, suggesting a predictive value that deserves prospective evaluation.
Keywords: COVID-19; S100A8; S100A9; SARS-CoV-2; calprotectin; emergency myelopoiesis; monocyte subsets; neutrophils; type I interferon.