tetano
Editor, Senior Moderator
Vaccine. 2015 Dec 4. pii: S0264-410X(15)01705-3. doi: 10.1016/j.vaccine.2015.11.055. [Epub ahead of print]
[h=1]Cell mediated immune responses following revaccination with an influenza A/H5N1 vaccine.[/h] Mbawuike IN[SUP]1[/SUP], Atmar RL[SUP]2[/SUP], Patel SM[SUP]3[/SUP], Corry DB[SUP]4[/SUP], Winokur PL[SUP]5[/SUP], Brady RC[SUP]6[/SUP], Chen WH[SUP]7[/SUP], Edwards KM[SUP]8[/SUP], Creech CB[SUP]8[/SUP], Walter EB Jr[SUP]9[/SUP], Frey SE[SUP]10[/SUP], Belshe RB[SUP]10[/SUP], Goll JB[SUP]11[/SUP], Hill H[SUP]11[/SUP], Keitel WA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]PURPOSE:[/h] The study aims were to determine whether inactivated influenza A/H5N1 vaccine administration elicited cell mediated immune (CMI) responses and the impact of adjuvant, vaccine dose and subject age on these responses.
[h=4]METHODS:[/h] Adults who were previously primed with either adjuvanted or unadjuvanted, inactivated, A/H5N1/Vietnam/1203/2004 (Clade 1) vaccine or unprimed (received placebo) in previous vaccine studies were randomized to receive one (primed) or two (unprimed) 15- or 90-mcg doses of inactivated, A/H5N1/Indonesia/05/05 (Clade 2) vaccine. Peripheral blood mononuclear cells (PBMCs) were collected and analyzed from a subset of vaccines to assess CMI responses using IFN-γ and granzyme B ELISPOT assays. Cytokine measurements were performed on PBMC supernatants after stimulation with H5N1 virus.
[h=4]RESULTS:[/h] PBMCs were available from 177 participants; 88 and 89 received 15-mcg and 90-mcg of unadjuvanted clade 2 vaccine, respectively. Following H5N1 clade 1 stimulation, IFN-γ but not granzyme B normalized spot-forming cell numbers had statistically significant increased numbers at each of the post-vaccination timepoints compared to baseline in pooled analyses of all vaccine doses and age groups. Clade 2 stimulation resulted in statistically significant increased numbers of IFN-γ cells only 180 days following the last vaccination. Responses were similar among younger and older study participants, as were responses among those primed with alum-adjuvanted or non-adjuvanted clade 1 H5N1 vaccines. The dosage of clade 2 vaccine did not impact CMI responses among primed subjects, but responses were statistically significantly greater in unprimed recipients of the 90-mcg dosage compared to unprimed recipients of the 15-mcg dosage. IFN-γ levels in the supernatants of stimulated PBMC were strongly correlated with IFN-γ ELISPOT results.
[h=4]CONCLUSION:[/h] CMI responses occur in adults administered influenza A/H5N1 inactivated influenza vaccine.
Copyright ? 2015 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Adjuvant; Cell mediated immune response; Cytokine; H5N1; Inactivated influenza vaccine
PMID: 26657997 [PubMed - as supplied by publisher]
[h=1]Cell mediated immune responses following revaccination with an influenza A/H5N1 vaccine.[/h] Mbawuike IN[SUP]1[/SUP], Atmar RL[SUP]2[/SUP], Patel SM[SUP]3[/SUP], Corry DB[SUP]4[/SUP], Winokur PL[SUP]5[/SUP], Brady RC[SUP]6[/SUP], Chen WH[SUP]7[/SUP], Edwards KM[SUP]8[/SUP], Creech CB[SUP]8[/SUP], Walter EB Jr[SUP]9[/SUP], Frey SE[SUP]10[/SUP], Belshe RB[SUP]10[/SUP], Goll JB[SUP]11[/SUP], Hill H[SUP]11[/SUP], Keitel WA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]PURPOSE:[/h] The study aims were to determine whether inactivated influenza A/H5N1 vaccine administration elicited cell mediated immune (CMI) responses and the impact of adjuvant, vaccine dose and subject age on these responses.
[h=4]METHODS:[/h] Adults who were previously primed with either adjuvanted or unadjuvanted, inactivated, A/H5N1/Vietnam/1203/2004 (Clade 1) vaccine or unprimed (received placebo) in previous vaccine studies were randomized to receive one (primed) or two (unprimed) 15- or 90-mcg doses of inactivated, A/H5N1/Indonesia/05/05 (Clade 2) vaccine. Peripheral blood mononuclear cells (PBMCs) were collected and analyzed from a subset of vaccines to assess CMI responses using IFN-γ and granzyme B ELISPOT assays. Cytokine measurements were performed on PBMC supernatants after stimulation with H5N1 virus.
[h=4]RESULTS:[/h] PBMCs were available from 177 participants; 88 and 89 received 15-mcg and 90-mcg of unadjuvanted clade 2 vaccine, respectively. Following H5N1 clade 1 stimulation, IFN-γ but not granzyme B normalized spot-forming cell numbers had statistically significant increased numbers at each of the post-vaccination timepoints compared to baseline in pooled analyses of all vaccine doses and age groups. Clade 2 stimulation resulted in statistically significant increased numbers of IFN-γ cells only 180 days following the last vaccination. Responses were similar among younger and older study participants, as were responses among those primed with alum-adjuvanted or non-adjuvanted clade 1 H5N1 vaccines. The dosage of clade 2 vaccine did not impact CMI responses among primed subjects, but responses were statistically significantly greater in unprimed recipients of the 90-mcg dosage compared to unprimed recipients of the 15-mcg dosage. IFN-γ levels in the supernatants of stimulated PBMC were strongly correlated with IFN-γ ELISPOT results.
[h=4]CONCLUSION:[/h] CMI responses occur in adults administered influenza A/H5N1 inactivated influenza vaccine.
Copyright ? 2015 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Adjuvant; Cell mediated immune response; Cytokine; H5N1; Inactivated influenza vaccine
PMID: 26657997 [PubMed - as supplied by publisher]