tetano
Editor, Senior Moderator
Cell Rep
. 2021 Dec 21;110235.
doi: 10.1016/j.celrep.2021.110235. Online ahead of print.
Cellular and humoral functional responses after BNT162b2 mRNA vaccination differ longitudinally between naive and subjects recovered from COVID-19
Roberto Lozano-Rodríguez[SUP] 1 [/SUP], Jaime Valentín-Quiroga[SUP] 1 [/SUP], José Avendaño-Ortiz[SUP] 1 [/SUP], Alejandro Martín-Quirós[SUP] 2 [/SUP], Alejandro Pascual-Iglesias[SUP] 1 [/SUP], Verónica Terrón-Arcos[SUP] 1 [/SUP], Karla Montalbán-Hernández[SUP] 1 [/SUP], José Carlos Casalvilla-Dueñas[SUP] 1 [/SUP], Marta Bergón-Gutiérrez[SUP] 1 [/SUP], José Alcamí[SUP] 3 [/SUP], Javier García-Pérez[SUP] 3 [/SUP], Almudena Cascajero[SUP] 3 [/SUP], Miguel Ángel García-Garrido[SUP] 2 [/SUP], Álvaro Del Balzo-Castillo[SUP] 4 [/SUP], María Peinado[SUP] 2 [/SUP], Laura Gómez[SUP] 2 [/SUP], Irene Llorente-Fernández[SUP] 5 [/SUP], Gema Martín-Miguel[SUP] 6 [/SUP], Carmen Herrero-Benito[SUP] 2 [/SUP], José Miguel Benito[SUP] 7 [/SUP], Norma Rallón[SUP] 7 [/SUP], Carmen Vela-Olmo[SUP] 8 [/SUP], Lissette López-Morejón[SUP] 8 [/SUP], Carolina Cubillos-Zapata[SUP] 9 [/SUP], Luis A Aguirre[SUP] 1 [/SUP], Carlos Del Fresno[SUP] 10 [/SUP], Eduardo López-Collazo[SUP] 11 [/SUP]
Affiliations
Abstract
We have analyzed BNT162b2 vaccine-induced immune responses in naive subjects and individuals recovered from coronavirus disease 2019 (COVID-19), both soon after (14 days) and later after (almost 8 months) vaccination. Plasma spike (S)-specific immunoglobulins peak after one vaccine shot in individuals recovered from COVID-19, while a second dose is needed in naive subjects, although the latter group shows reduced levels all along the analyzed period. Despite how the neutralization capacity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mirrors this behavior early after vaccination, both groups show comparable neutralizing antibodies and S-specific B cell levels late post-vaccination. When studying cellular responses, naive individuals exhibit higher SARS-CoV-2-specific cytokine production, CD4[SUP]+[/SUP] T cell activation, and proliferation than do individuals recovered from COVID-19, with patent inverse correlations between humoral and cellular variables early post-vaccination. However, almost 8 months post-vaccination, SARS-CoV-2-specific responses are comparable between both groups. Our data indicate that a previous history of COVID-19 differentially determines the functional T and B cell-mediated responses to BNT162b2 vaccination over time.
Keywords: BNT162b2 vaccine; COVID-19-recovered; SARS-CoV-2; antigen-specific T cell; cellular responses; cytokine production; humoral responses; memory B cell; proliferation; spike.
. 2021 Dec 21;110235.
doi: 10.1016/j.celrep.2021.110235. Online ahead of print.
Cellular and humoral functional responses after BNT162b2 mRNA vaccination differ longitudinally between naive and subjects recovered from COVID-19
Roberto Lozano-Rodríguez[SUP] 1 [/SUP], Jaime Valentín-Quiroga[SUP] 1 [/SUP], José Avendaño-Ortiz[SUP] 1 [/SUP], Alejandro Martín-Quirós[SUP] 2 [/SUP], Alejandro Pascual-Iglesias[SUP] 1 [/SUP], Verónica Terrón-Arcos[SUP] 1 [/SUP], Karla Montalbán-Hernández[SUP] 1 [/SUP], José Carlos Casalvilla-Dueñas[SUP] 1 [/SUP], Marta Bergón-Gutiérrez[SUP] 1 [/SUP], José Alcamí[SUP] 3 [/SUP], Javier García-Pérez[SUP] 3 [/SUP], Almudena Cascajero[SUP] 3 [/SUP], Miguel Ángel García-Garrido[SUP] 2 [/SUP], Álvaro Del Balzo-Castillo[SUP] 4 [/SUP], María Peinado[SUP] 2 [/SUP], Laura Gómez[SUP] 2 [/SUP], Irene Llorente-Fernández[SUP] 5 [/SUP], Gema Martín-Miguel[SUP] 6 [/SUP], Carmen Herrero-Benito[SUP] 2 [/SUP], José Miguel Benito[SUP] 7 [/SUP], Norma Rallón[SUP] 7 [/SUP], Carmen Vela-Olmo[SUP] 8 [/SUP], Lissette López-Morejón[SUP] 8 [/SUP], Carolina Cubillos-Zapata[SUP] 9 [/SUP], Luis A Aguirre[SUP] 1 [/SUP], Carlos Del Fresno[SUP] 10 [/SUP], Eduardo López-Collazo[SUP] 11 [/SUP]
Affiliations
- PMID: 34986327
- DOI: 10.1016/j.celrep.2021.110235
Abstract
We have analyzed BNT162b2 vaccine-induced immune responses in naive subjects and individuals recovered from coronavirus disease 2019 (COVID-19), both soon after (14 days) and later after (almost 8 months) vaccination. Plasma spike (S)-specific immunoglobulins peak after one vaccine shot in individuals recovered from COVID-19, while a second dose is needed in naive subjects, although the latter group shows reduced levels all along the analyzed period. Despite how the neutralization capacity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mirrors this behavior early after vaccination, both groups show comparable neutralizing antibodies and S-specific B cell levels late post-vaccination. When studying cellular responses, naive individuals exhibit higher SARS-CoV-2-specific cytokine production, CD4[SUP]+[/SUP] T cell activation, and proliferation than do individuals recovered from COVID-19, with patent inverse correlations between humoral and cellular variables early post-vaccination. However, almost 8 months post-vaccination, SARS-CoV-2-specific responses are comparable between both groups. Our data indicate that a previous history of COVID-19 differentially determines the functional T and B cell-mediated responses to BNT162b2 vaccination over time.
Keywords: BNT162b2 vaccine; COVID-19-recovered; SARS-CoV-2; antigen-specific T cell; cellular responses; cytokine production; humoral responses; memory B cell; proliferation; spike.