tetano
Editor, Senior Moderator
Cell Rep
. 2023 Apr 21;42(5):112470.
doi: 10.1016/j.celrep.2023.112470. Online ahead of print. Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination
Zixi Yin[SUP] 1 [/SUP], Ji-Li Chen[SUP] 1 [/SUP], Yongxu Lu[SUP] 2 [/SUP], Beibei Wang[SUP] 3 [/SUP], Leila Godfrey[SUP] 4 [/SUP], Alexander J Mentzer[SUP] 5 [/SUP], Xuan Yao[SUP] 3 [/SUP], Guihai Liu[SUP] 6 [/SUP], Dannielle Wellington[SUP] 3 [/SUP], Yiqi Zhao[SUP] 2 [/SUP], Peter A C Wing[SUP] 7 [/SUP], Wanwisa Dejnirattisa[SUP] 8 [/SUP], Piyada Supasa[SUP] 5 [/SUP], Chang Liu[SUP] 9 [/SUP], Philip Hublitz[SUP] 10 [/SUP], Ryan Beveridge[SUP] 11 [/SUP], Craig Waugh[SUP] 12 [/SUP], Sally-Ann Clark[SUP] 12 [/SUP], Kevin Clark[SUP] 12 [/SUP], Paul Sopp[SUP] 12 [/SUP], Timothy Rostron[SUP] 13 [/SUP], Juthathip Mongkolsapaya[SUP] 9 [/SUP], Gavin R Screaton[SUP] 9 [/SUP], Graham Ogg[SUP] 1 [/SUP], Katie Ewer[SUP] 14 [/SUP], Andrew J Pollard[SUP] 15 [/SUP], Sarah Gilbert[SUP] 16 [/SUP], Julian C Knight[SUP] 9 [/SUP], Teresa Lambe[SUP] 17 [/SUP], Geoffrey L Smith[SUP] 18 [/SUP], Tao Dong[SUP] 19 [/SUP], Yanchun Peng[SUP] 20 [/SUP]
Affiliations
Most existing studies characterizing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses are peptide based. This does not allow evaluation of whether tested peptides are processed and presented canonically. In this study, we use recombinant vaccinia virus (rVACV)-mediated expression of SARS-CoV-2 spike protein and SARS-CoV-2 infection of angiotensin-converting enzyme (ACE)-2-transduced B cell lines to evaluate overall T cell responses in a small cohort of recovered COVID-19 patients and uninfected donors vaccinated with ChAdOx1 nCoV-19. We show that rVACV expression of SARS-CoV-2 antigen can be used as an alternative to SARS-CoV-2 infection to evaluate T cell responses to naturally processed spike antigens. In addition, the rVACV system can be used to evaluate the cross-reactivity of memory T cells to variants of concern (VOCs) and to identify epitope escape mutants. Finally, our data show that both natural infection and vaccination could induce multi-functional T cell responses with overall T cell responses remaining despite the identification of escape mutations.
Keywords: CP: Immunology; ChAdOx1 nCoV-19; SARS-CoV-2; T cell; antigen processing and presentation; immune escape; recombinant vaccinia virus; spike protein; variants of concern.
. 2023 Apr 21;42(5):112470.
doi: 10.1016/j.celrep.2023.112470. Online ahead of print. Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination
Zixi Yin[SUP] 1 [/SUP], Ji-Li Chen[SUP] 1 [/SUP], Yongxu Lu[SUP] 2 [/SUP], Beibei Wang[SUP] 3 [/SUP], Leila Godfrey[SUP] 4 [/SUP], Alexander J Mentzer[SUP] 5 [/SUP], Xuan Yao[SUP] 3 [/SUP], Guihai Liu[SUP] 6 [/SUP], Dannielle Wellington[SUP] 3 [/SUP], Yiqi Zhao[SUP] 2 [/SUP], Peter A C Wing[SUP] 7 [/SUP], Wanwisa Dejnirattisa[SUP] 8 [/SUP], Piyada Supasa[SUP] 5 [/SUP], Chang Liu[SUP] 9 [/SUP], Philip Hublitz[SUP] 10 [/SUP], Ryan Beveridge[SUP] 11 [/SUP], Craig Waugh[SUP] 12 [/SUP], Sally-Ann Clark[SUP] 12 [/SUP], Kevin Clark[SUP] 12 [/SUP], Paul Sopp[SUP] 12 [/SUP], Timothy Rostron[SUP] 13 [/SUP], Juthathip Mongkolsapaya[SUP] 9 [/SUP], Gavin R Screaton[SUP] 9 [/SUP], Graham Ogg[SUP] 1 [/SUP], Katie Ewer[SUP] 14 [/SUP], Andrew J Pollard[SUP] 15 [/SUP], Sarah Gilbert[SUP] 16 [/SUP], Julian C Knight[SUP] 9 [/SUP], Teresa Lambe[SUP] 17 [/SUP], Geoffrey L Smith[SUP] 18 [/SUP], Tao Dong[SUP] 19 [/SUP], Yanchun Peng[SUP] 20 [/SUP]
Affiliations
- PMID: 37141092
- DOI: 10.1016/j.celrep.2023.112470
Most existing studies characterizing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses are peptide based. This does not allow evaluation of whether tested peptides are processed and presented canonically. In this study, we use recombinant vaccinia virus (rVACV)-mediated expression of SARS-CoV-2 spike protein and SARS-CoV-2 infection of angiotensin-converting enzyme (ACE)-2-transduced B cell lines to evaluate overall T cell responses in a small cohort of recovered COVID-19 patients and uninfected donors vaccinated with ChAdOx1 nCoV-19. We show that rVACV expression of SARS-CoV-2 antigen can be used as an alternative to SARS-CoV-2 infection to evaluate T cell responses to naturally processed spike antigens. In addition, the rVACV system can be used to evaluate the cross-reactivity of memory T cells to variants of concern (VOCs) and to identify epitope escape mutants. Finally, our data show that both natural infection and vaccination could induce multi-functional T cell responses with overall T cell responses remaining despite the identification of escape mutations.
Keywords: CP: Immunology; ChAdOx1 nCoV-19; SARS-CoV-2; T cell; antigen processing and presentation; immune escape; recombinant vaccinia virus; spike protein; variants of concern.