tetano
Editor, Senior Moderator
Cell Rep
. 2020 Jul 3;107918.
doi: 10.1016/j.celrep.2020.107918. Online ahead of print.
Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infection
Jinkai Wan[SUP] 1 [/SUP], Shenghui Xing[SUP] 1 [/SUP], Longfei Ding[SUP] 2 [/SUP], Yongheng Wang[SUP] 3 [/SUP], Chenjian Gu[SUP] 4 [/SUP], Yanling Wu[SUP] 4 [/SUP], Bowen Rong[SUP] 1 [/SUP], Cheng Li[SUP] 4 [/SUP], Siqing Wang[SUP] 1 [/SUP], Kun Chen[SUP] 1 [/SUP], Chenxi He[SUP] 1 [/SUP], Dandan Zhu[SUP] 5 [/SUP], Songhua Yuan[SUP] 2 [/SUP], Chengli Qiu[SUP] 2 [/SUP], Chen Zhao[SUP] 2 [/SUP], Lei Nie[SUP] 6 [/SUP], Zhangzhao Gao[SUP] 6 [/SUP], Jingyu Jiao[SUP] 6 [/SUP], Xiaoyan Zhang[SUP] 7 [/SUP], Xiangxi Wang[SUP] 5 [/SUP], Tianlei Ying[SUP] 4 [/SUP], Haibin Wang[SUP] 6 [/SUP], Youhua Xie[SUP] 8 [/SUP], Yanan Lu[SUP] 9 [/SUP], Jianqing Xu[SUP] 10 [/SUP], Fei Lan[SUP] 11 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) has become a worldwide threat to humans, and neutralizing antibodies have therapeutic potential. We have purified more than 1,000 memory B cells specific to SARS-CoV-2 S1 or its RBD (receptor binding domain) and obtain 729 paired heavy- and light-chain fragments. Among these, 178 antibodies test positive for antigen binding, and the majority of the top 17 binders with EC[SUB]50[/SUB] below 1 nM are RBD binders. Furthermore, we identify 11 neutralizing antibodies, eight of which show IC[SUB]50[/SUB] within 10 nM, and the best one, 414-1, with IC[SUB]50[/SUB] of 1.75 nM. Through epitope mapping, we find three main epitopes in RBD recognized by these antibodies, and epitope-B antibody 553-15 could substantially enhance the neutralizing abilities of most of the other antibodies. We also find that 515-5 could cross neutralize the SARS-CoV pseudovirus. Altogether, our study provides 11 potent human neutralizing antibodies for COVID-19 as therapeutic candidates.
Keywords: COVID-19; RBD; SARS-CoV-2; coronavirus; cross-neutralizing antibody; epitope; human antibodies; infection; neutralizing antibodies; spike protein.
. 2020 Jul 3;107918.
doi: 10.1016/j.celrep.2020.107918. Online ahead of print.
Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infection
Jinkai Wan[SUP] 1 [/SUP], Shenghui Xing[SUP] 1 [/SUP], Longfei Ding[SUP] 2 [/SUP], Yongheng Wang[SUP] 3 [/SUP], Chenjian Gu[SUP] 4 [/SUP], Yanling Wu[SUP] 4 [/SUP], Bowen Rong[SUP] 1 [/SUP], Cheng Li[SUP] 4 [/SUP], Siqing Wang[SUP] 1 [/SUP], Kun Chen[SUP] 1 [/SUP], Chenxi He[SUP] 1 [/SUP], Dandan Zhu[SUP] 5 [/SUP], Songhua Yuan[SUP] 2 [/SUP], Chengli Qiu[SUP] 2 [/SUP], Chen Zhao[SUP] 2 [/SUP], Lei Nie[SUP] 6 [/SUP], Zhangzhao Gao[SUP] 6 [/SUP], Jingyu Jiao[SUP] 6 [/SUP], Xiaoyan Zhang[SUP] 7 [/SUP], Xiangxi Wang[SUP] 5 [/SUP], Tianlei Ying[SUP] 4 [/SUP], Haibin Wang[SUP] 6 [/SUP], Youhua Xie[SUP] 8 [/SUP], Yanan Lu[SUP] 9 [/SUP], Jianqing Xu[SUP] 10 [/SUP], Fei Lan[SUP] 11 [/SUP]
Affiliations
- PMID: 32668215
- DOI: 10.1016/j.celrep.2020.107918
Abstract
Coronavirus disease 2019 (COVID-19) has become a worldwide threat to humans, and neutralizing antibodies have therapeutic potential. We have purified more than 1,000 memory B cells specific to SARS-CoV-2 S1 or its RBD (receptor binding domain) and obtain 729 paired heavy- and light-chain fragments. Among these, 178 antibodies test positive for antigen binding, and the majority of the top 17 binders with EC[SUB]50[/SUB] below 1 nM are RBD binders. Furthermore, we identify 11 neutralizing antibodies, eight of which show IC[SUB]50[/SUB] within 10 nM, and the best one, 414-1, with IC[SUB]50[/SUB] of 1.75 nM. Through epitope mapping, we find three main epitopes in RBD recognized by these antibodies, and epitope-B antibody 553-15 could substantially enhance the neutralizing abilities of most of the other antibodies. We also find that 515-5 could cross neutralize the SARS-CoV pseudovirus. Altogether, our study provides 11 potent human neutralizing antibodies for COVID-19 as therapeutic candidates.
Keywords: COVID-19; RBD; SARS-CoV-2; coronavirus; cross-neutralizing antibody; epitope; human antibodies; infection; neutralizing antibodies; spike protein.